Department of Hygiene and Epidemiology, Medical University of Lublin, Chodzki 7 Street, 20-093, Lublin, Poland.
Military Medical Institute, Szaserów 128 Street, 04-141, Warszawa, Poland.
BMC Nephrol. 2022 May 28;23(1):198. doi: 10.1186/s12882-022-02828-1.
Pyrethroids are used for insect control. They act on voltage-gated sodium channels in neurons. Lambdacyhalothrin (LCH) is a type II pyrethroid producing choreoatetosis and salivation in rodents. Vascular endothelial growth factor (VEGF) expression in the kidney is high in the glomerular podocytes and kidney tubules. VEGF receptor 2 (VEFGR2) is the main mediator in angiogenesis and it regulates blood vessel permeability. Lack of VEGF in podocytes impairs filtration. The nuclear factor κB (NFκB) is widely known as an important mediator of inflammation. The aim of the study was to check if subacute oral intoxication with 0.1LD of LCH affects kidney size, function and VEGFR2 and NFκB in mice kidneys.
A total of 32 Albino Swiss mice was used: females controls, males controls, females receiving 2 mg/kg LCH, males receiving 2 mg/kg LCH orally for 7 days. On day 8 animals were sacrificed, blood and kidneys were obtained. Kidney mass was determined, creatinine concentration was measured in blood sera, VEGFR2 and NFκB in kidney homogenate supernatant with ELISA kit.
There was no statistically significant differences in kidney mass, creatinine concentration in blood sera nor NFκB but mean VEGFR2 concentration in the kidneys of females exposed to LCH was 128.01 ng/ml and showed statistically significant difference in comparison with control females. There was no statistically significant difference between VEGFR2 concentration in the kidneys of males exposed to LCH and control males.
The VEGFR2 increases in the course of LCH intoxication in females probably due to the protective effect of oestrogens.
拟除虫菊酯用于昆虫控制。它们作用于神经元中的电压门控钠离子通道。溴氰菊酯（LCH）是一种 II 型拟除虫菊酯，会导致啮齿动物出现舞蹈病和流涎。肾脏中的血管内皮生长因子（VEGF）在肾小球足细胞和肾小管中表达较高。血管内皮生长因子受体 2（VEFGR2）是血管生成的主要介质，它调节血管通透性。足细胞中缺乏 VEGF 会损害过滤功能。核因子 κB（NFκB）被广泛认为是炎症的重要介质。本研究旨在检查亚急性口服 0.1LD 的 LCH 对小鼠肾脏大小、功能以及 VEGFR2 和 NFκB 的影响。
共使用 32 只白化瑞士小鼠：雌性对照组、雄性对照组、雌性给予 2mg/kg LCH、雄性给予 2mg/kg LCH 连续 7 天口服。第 8 天处死动物，获取血液和肾脏。测定肾脏质量，检测血清肌酐浓度，采用 ELISA 试剂盒检测肾脏匀浆上清液中的 VEGFR2 和 NFκB。
LCH 暴露的雌性动物肾脏质量、血清肌酐浓度或 NFκB 均无统计学显著差异，但暴露于 LCH 的雌性动物肾脏中 VEGFR2 的平均浓度为 128.01ng/ml，与对照组雌性动物相比具有统计学显著差异。暴露于 LCH 的雄性动物肾脏中 VEGFR2 的浓度与对照组雄性动物之间无统计学显著差异。
LCH 中毒过程中雌性动物 VEGFR2 的增加可能是由于雌激素的保护作用。
