Namba Andressa Mayumi, Santos Evelyn Luzia de Souza, Garcia Maíra Terra, Ribeiro Felipe de Camargo, Figueiredo-Godoi Lívia Mara Alves, Rossoni Rodnei Dennis, Junqueira Juliana Campos
Department of Biosciences and Oral Diagnosis, Institute of Science and Technology of São Paulo State University/UNESP, Av. Francisco José Longo 777, São Dimas, São José dos Campos, SP 12245-000, Brazil.
Department of Biosciences and Oral Diagnosis, Institute of Science and Technology of São Paulo State University/UNESP, Av. Francisco José Longo 777, São Dimas, São José dos Campos, SP 12245-000, Brazil.
Photodiagnosis Photodyn Ther. 2022 Sep;39:102928. doi: 10.1016/j.pdpdt.2022.102928. Epub 2022 May 26.
Enterococcus faecalis is related to the recurrence of endodontic infections and approaches to intracanal disinfection are necessary. Farnesol, an alcohol commonly found in propolis, has antimicrobial properties, and can enhance the efficacy of some antibiotic therapies. The objective was to evaluate whether farnesol can increase the efficacy of the antimicrobial photodynamic inactivation (aPDI) on E. faecalis, investigating its action on planktonic growth, biofilms, and cell permeability. Planktonic cells and biofilms of E. faecalis were pre-treated with farnesol (0.25 mM) 2 h before aPDI. Methylene blue (1 mg/mL) and laser (660 nm) were employed in the aPDI. As a result, farnesol was able to increase the antimicrobial activity of aPDI in both planktonic and biofilm stages, reaching cell reductions of 4.6 to 6 log10 CFU and 1.3 to 3 log10 CFU, respectively, when compared to aPDI isolated. The efficacy of farnesol in enhancing the anti-biofilm activity of aPDI was also confirmed by electron microscopy, in which a smaller number of bacterial cells and extracellular matrix were verified in the combined therapy compared to aPDI alone. The potentiating action of farnesol was associated with its effects in increasing the cell permeability and methylene blue uptake by the bacterial cells. Therefore, farnesol can be a promising potentiator of aPDI against E. faecalis.
粪肠球菌与牙髓感染的复发有关，因此有必要采取根管内消毒方法。法尼醇是蜂胶中常见的一种醇类，具有抗菌特性，并且可以增强某些抗生素疗法的疗效。本研究的目的是评估法尼醇是否能提高抗菌光动力灭活（aPDI）对粪肠球菌的疗效，研究其对浮游菌生长、生物膜及细胞通透性的作用。在进行aPDI前2小时，用0.25 mM法尼醇对粪肠球菌的浮游菌细胞和生物膜进行预处理。aPDI采用亚甲蓝（1 mg/mL）和激光（660 nm）。结果显示，与单独使用aPDI相比，法尼醇能够在浮游菌和生物膜阶段提高aPDI的抗菌活性，使细胞减少量分别达到4.6至6个对数10 CFU和1.3至3个对数10 CFU。电子显微镜检查也证实了法尼醇在增强aPDI抗生物膜活性方面的功效，与单独使用aPDI相比，联合治疗中观察到的细菌细胞和细胞外基质数量较少。法尼醇的增强作用与其增加细菌细胞通透性和亚甲蓝摄取量的作用有关。因此，法尼醇可能是一种有前景的aPDI增强剂，可用于对抗粪肠球菌。
