Gao Han, Gao Chen-Chen, Wang Tian-Tian, Gao Lei, Li Guang-Wen, Jin Liang-Yun, He Cheng-Wei, Wang Bo-Ya, Zhang Lucia, Guo Yue-Xin, Hua Rong-Xuan, Shang Hong-Wei, Xu Jing-Dong
Department of Physiology and Pathophysiology, Basic Medical College, Capital Medical University, Beijing 100069, China.
Department of Biomedical Informatics, School of Biomedical Engineering, Capital Medical University, Beijing 100069, China.
Microsc Microanal. 2022 May 30:1-14. doi: 10.1017/S1431927622000836.
Due to the lack of research between the inner layers in the structure of colonic mucous and the metabolism of fatty acid in the constipation model, we aim to determine the changes in the mucous phenotype of the colonic glycocalyx and the microbial community structure following treatment with Rhubarb extract in our research. The constipation and treatment models are generated using adult male C57BL/6N mice. We perform light microscopy and transmission electron microscopy (TEM) to detect a Muc2-rich inner mucus layer attached to mice colon under different conditions. In addition, 16S rDNA sequencing is performed to examine the intestinal flora. According to TEM images, we demonstrate that Rhubarb can promote mucin secretion and find direct evidence of dendritic structure-linked mucus structures with its assembly into a lamellar network in a pore size distribution in the isolated colon section. Moreover, the diversity of intestinal flora has noticeable changes in constipated mice. The present study characterizes a dendritic structure and persistent cross-links have significant changes accompanied by the alteration of intestinal flora in feces in models of constipation and pretreatment with Rhubarb extract.
由于在便秘模型中结肠黏液结构内层与脂肪酸代谢之间缺乏研究，我们旨在通过本研究确定用大黄提取物处理后结肠糖萼黏液表型和微生物群落结构的变化。便秘和治疗模型采用成年雄性C57BL/6N小鼠构建。我们进行光学显微镜和透射电子显微镜（TEM）检测不同条件下小鼠结肠附着的富含Muc2的内黏液层。此外，进行16S rDNA测序以检查肠道菌群。根据TEM图像，我们证明大黄可促进黏蛋白分泌，并在分离的结肠段孔径分布中发现其组装成层状网络的树突状结构连接黏液结构的直接证据。此外，便秘小鼠的肠道菌群多样性有明显变化。本研究表明，在便秘模型和大黄提取物预处理中，粪便中树突状结构和持久交联伴随肠道菌群改变发生了显著变化。
