Centro de Referencia para Lactobacilos (CERELA-CCT NOA Sur-CONICET), Chacabuco 145, T4000ILC, San Miguel de Tucumán, Tucumán, Argentina; Centro Científico Tecnológico NOA Sur - CONICET, Crisóstomo Álvarez 722, T4000ILC, San Miguel de Tucumán, Tucumán, Argentina.
Centro Científico Tecnológico NOA Sur - CONICET, Crisóstomo Álvarez 722, T4000ILC, San Miguel de Tucumán, Tucumán, Argentina; Universidad Nacional de Tucumán, Ayacucho 491, T4000INI, San Miguel de Tucumán, Tucumán, Argentina.
Rev Argent Microbiol. 2022 Oct-Dec;54(4):263-267. doi: 10.1016/j.ram.2022.02.006. Epub 2022 May 26.
Acidipropionibacterium acidipropionici is widely used for many applications, such as propionic acid production, cereal silage, and also as probiotic. Due to this plethora of applications, new isolates of A. acidipropionici with improved features are being searched for. These new isolates must be accurately identified, however, most approaches become expensive and time-consuming when the number of isolates is high. On the contrary, fluorescence in situ hybridization allows the affordable, reliable, and rapid identification of microorganisms in pure cultures and environmental and medical samples. Therefore, the aim of this work was to apply a fluorescent in situ hybridization probe for the reliable identification of new A. acidipropionici isolates. To this end, probe Pap446, specific for A. acidipropionici, was validated by hybridization assays with strains of this species from different origins, other species of the same genus or family, and unrelated genera. Eight isolates with propionibacterium characteristics were obtained from milk and feces of cows. Probe Pap446, hybridized only with isolates III and VI. The identity of these isolates was further confirmed by PCR using group and species-specific primers for propionibacteria and 16S rDNA sequencing.
丙酸短杆菌被广泛应用于许多领域，例如丙酸生产、谷物青贮饲料，也被用作益生菌。由于其广泛的应用，人们正在寻找具有改进特性的新型丙酸短杆菌分离株。然而，当分离株数量较高时，大多数方法的准确性和速度都会受到影响，变得既昂贵又耗时。相比之下，荧光原位杂交技术可以在纯培养物、环境和医学样本中实现微生物的经济、可靠和快速鉴定。因此，本研究旨在应用荧光原位杂交探针来可靠鉴定新型丙酸短杆菌分离株。为此，通过与来自不同来源的该种菌株、同属或同科的其他种菌株以及不相关的属菌株进行杂交实验，对特异性针对丙酸短杆菌的探针 Pap446 进行了验证。从牛奶和奶牛粪便中获得了 8 株具有丙酸杆菌特征的分离株。只有分离株 III 和 VI 与探针 Pap446 杂交。通过使用针对丙酸杆菌的属和种特异性引物以及 16S rDNA 测序对这些分离株进行 PCR 进一步确认了它们的身份。
