Deptula Paulina, Smolander Olli-Pekka, Laine Pia, Roberts Richard J, Edelmann Minnamari, Peltola Petri, Piironen Vieno, Paulin Lars, Storgårds Erna, Savijoki Kirsi, Laitila Arja, Auvinen Petri, Varmanen Pekka
1Department of Food and Nutrition, University of Helsinki, 00014 Helsinki, Finland.
2Institute of Biotechnology, University of Helsinki, 00014 Helsinki, Finland.
Int J Syst Evol Microbiol. 2018 Oct;68(10):3175-3183. doi: 10.1099/ijsem.0.002965. Epub 2018 Aug 29.
A Gram-stain-positive, catalase-positive and pleomorphic rod organism was isolated from malted barley in Finland, classified initially by partial 16S rRNA gene sequencing and originally deposited in the VTT Culture Collection as a strain of Propionibacterium acidipropionici (currently Acidipropionibacterium acidipropionici). The subsequent comparison of the whole 16S rRNA gene with other representatives of the genus Acidipropionibacterium revealed that the strain belongs to a novel species, most closely related to Acidipropionibacterium microaerophilum and Acidipropionibacterium acidipropionici, with similarity values of 98.46 and 98.31 %, respectively. The whole genome sequencing using PacBio RS II platform allowed further comparison of the genome with all of the other DNA sequences available for the type strains of the Acidipropionibacterium species. Those comparisons revealed the highest similarity of strain JS278 to A. acidipropionici, which was confirmed by the average nucleotide identity analysis. The genome of strain JS278 is intermediate in size compared to the A. acidipropionici and Acidipropionibacterium jensenii at 3 432 872 bp, the G+C content is 68.4 mol%. The strain fermented a wide range of carbon sources, and produced propionic acid as the major fermentation product. Besides its poor ability to grow at 37 °C and positive catalase reaction, the observed phenotype was almost indistinguishable from those of A. acidipropionici and A. jensenii. Based on our findings, we conclude that the organism represents a novel member of the genus Acidipropionibacterium, for which we propose the name Acidipropionibacteriumvirtanenii sp. nov. The type strain is JS278 (=VTT E-113202=DSM 106790).
从芬兰的麦芽大麦中分离出一种革兰氏染色阳性、过氧化氢酶阳性且形态多样的杆状微生物，最初通过部分16S rRNA基因测序进行分类，最初作为丙酸丙酸杆菌（现为酸丙酸丙酸杆菌）的一个菌株保藏于VTT菌种保藏中心。随后将该菌株的完整16S rRNA基因与酸丙酸丙酸杆菌属的其他代表菌株进行比较，结果表明该菌株属于一个新物种，与嗜微氧酸丙酸丙酸杆菌和酸丙酸丙酸杆菌关系最为密切，相似性值分别为98.46%和98.31%。使用PacBio RS II平台进行全基因组测序，使得能够进一步将该基因组与酸丙酸丙酸杆菌属模式菌株的所有其他可用DNA序列进行比较。这些比较显示菌株JS278与酸丙酸丙酸杆菌的相似性最高，平均核苷酸同一性分析证实了这一点。与酸丙酸丙酸杆菌和詹氏酸丙酸丙酸杆菌相比，菌株JS278的基因组大小处于中间水平，为3432872 bp，G+C含量为68.4 mol%。该菌株能发酵多种碳源，并产生丙酸作为主要发酵产物。除了在37°C下生长能力较差和过氧化氢酶反应呈阳性外，观察到的表型与酸丙酸丙酸杆菌和詹氏酸丙酸丙酸杆菌的表型几乎无法区分。基于我们的研究结果，我们得出结论，该微生物代表酸丙酸丙酸杆菌属的一个新成员，我们将其命名为维塔宁酸丙酸丙酸杆菌（Acidipropionibacterium virtanenii sp. nov.）。模式菌株为JS278（=VTT E-113202=DSM 106790）。
