Division of Applied Life Science (BK21+) and PMBBRC, Gyeongsang National University, Jinju, Korea.
College of Pharmacy, Gyeongsang National University, Jinju, Korea.
FEBS Lett. 2022 Aug;596(15):1871-1880. doi: 10.1002/1873-3468.14410. Epub 2022 Jun 16.
Environmental stresses restrict plant growth and development and decrease crop yield. The circadian clock is associated with the ability of a plant to adapt to daily environmental fluctuations and the production and consumption of energy. Here, we investigated the role of Arabidopsis Universal Stress Protein (USP; At3g53990) in the circadian regulation of nuclear clock genes. The Arabidopsis usp knockout mutant line exhibited critically diminished circadian amplitude of the central oscillator CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) but enhanced the amplitude of TIMING OF CAB EXPRESSION 1 (TOC1). However, the expression of USP under the control of its own promoter restored the circadian timing of both genes, suggesting that USP regulates the circadian rhythm of Arabidopsis central clock genes, CCA1 and TOC1.
环境胁迫限制植物的生长和发育，降低作物产量。生物钟与植物适应每日环境波动以及能量的产生和消耗的能力有关。在这里，我们研究了拟南芥泛素特异性蛋白酶（USP；At3g53990）在核钟基因的生物钟调节中的作用。拟南芥usp 敲除突变体表现出中央振荡器 CIRCADIAN CLOCK ASSOCIATED 1（CCA1）的生物钟振幅显著减小，但增强了 TIMING OF CAB EXPRESSION 1（TOC1）的振幅。然而，在其自身启动子的控制下表达 USP 恢复了这两个基因的生物钟定时，表明 USP 调节拟南芥中央生物钟基因 CCA1 和 TOC1 的生物钟节律。
