高尔基磷酸蛋白 3 促进卵巢癌进展，并与顺铂耐药相关。

Golgi phosphoprotein 3 promotes ovarian cancer progression and is associated with cisplatin resistance.

机构信息

Department of Obstetrics and Gynecology, The First Affiliated Hospital of Shandong First Medical University and Shandong Provincial Qianfoshan Hospital, Key Laboratory of Laparoscopic Technology, The First Affiliated Hospital of Shandong First Medical University; Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, Shandong, P.R. China.

出版信息

J Cancer Res Ther. 2022 Apr;18(2):488-495. doi: 10.4103/jcrt.jcrt_2348_21.

DOI:10.4103/jcrt.jcrt_2348_21

PMID:35645119

Abstract

BACKGROUND

Golgi phosphoprotein-3 (GOLPH 3) is involved in the development of several human cancers. However, the clinical significance and biological role of GOLPH 3 in ovarian cancer (OC) remains unknown.

METHODS

The expression of GOLPH 3 in OC cell lines was quantified using real-time quantitative polymerase chain reaction (RT-qPCR) and western blot assays. The role of GOLPH 3 in tumorigenicity, migration, and invasion of OC cell lines by small interference RNA, scratch wound-healing assays, and transwell assays was detected. In addition, western blotting was used to determine whether GOLPH 3 is associated with the PI3K/AKT/mTOR signaling pathway. Furthermore, RT-qPCR verified whether GOLPH 3 is associated with drug resistance.

RESULTS

GOLPH 3-positive expression rate was higher in OC. Downregulation of GOLPH 3 markedly inhibited the migration and invasion and may be related to the PI3K/AKT/mTOR signal pathway. Moreover, the result of the experiment proved that GOLPH 3 enhances the sensitivity of OC to cisplatin by regulating ATP7A/B. GOLPH 3 promoted the invasion and migration of OC, and the mechanism may be related to the PI3K/Akt/mTOR pathway. In addition, inhibition of GOLPH 3 increased the sensitivity of OC cells to cisplatin, which may be associated with ATP7A/B.

CONCLUSION

This study found that GOLPH3 may promote the migration and invasion of OC cells through PI3K/Akt/mTOR pathway. At the same time, low expression of GOLPH3 increased the sensitivity of OC cells to cisplatin.

摘要

背景

高尔基磷酸蛋白 3（GOLPH3）参与了多种人类癌症的发生。然而，GOLPH3 在卵巢癌（OC）中的临床意义和生物学作用尚不清楚。

方法

采用实时定量聚合酶链反应（RT-qPCR）和 Western blot 检测 OC 细胞系中 GOLPH3 的表达。通过小干扰 RNA、划痕愈合实验和 Transwell 实验检测 GOLPH3 对 OC 细胞系致瘤性、迁移和侵袭的作用。此外，Western blot 用于确定 GOLPH3 是否与 PI3K/AKT/mTOR 信号通路相关。此外，RT-qPCR 验证 GOLPH3 是否与耐药性相关。

结果

GOLPH3 在 OC 中呈阳性表达率较高。下调 GOLPH3 明显抑制迁移和侵袭，可能与 PI3K/AKT/mTOR 信号通路有关。此外，实验结果证明，GOLPH3 通过调节 ATP7A/B 增强了 OC 对顺铂的敏感性。GOLPH3 促进 OC 的侵袭和迁移，其机制可能与 PI3K/Akt/mTOR 通路有关。此外，抑制 GOLPH3 增加了 OC 细胞对顺铂的敏感性，这可能与 ATP7A/B 有关。