Rincón-Riveros Andrés, Rodríguez Josefa Antonia, Villegas Victoria E, López-Kleine Liliana
Bioinformatics and Systems Biology Group, Universidad Nacional de Colombia, Bogotá 111221, Colombia.
Cancer Biology Research Group, Instituto Nacional de Cancerología, Bogotá 111221, Colombia.
Noncoding RNA. 2022 May 12;8(3):33. doi: 10.3390/ncrna8030033.
Exosomes carry molecules of great biological and clinical interest, such as miRNAs. The contents of exosomes vary between healthy controls and cancer patients. Therefore, miRNAs and other molecules transported in exosomes are considered a potential source of diagnostic and prognostic biomarkers in cancer. Many miRNAs have been detected in recent years. Consequently, a substantial amount of miRNA-related data comparing patients and healthy individuals is available, which contributes to a better understanding of the initiation, development, malignancy, and metastasis of cancer using non-invasive sampling procedures. However, a re-analysis of available ncRNA data is rare. This study used available data about miRNAs in exosomes comparing healthy individuals and cancer patients to identify possible global changes related to the presence of cancer. A robust transcriptomic analysis identified two common miRNAs (miR-495-3p and miR-543) deregulated in five cancer datasets. They had already been implicated in different cancers but not reported in exosomes circulating in blood. The study also examined their target genes and the implications of these genes for functional processes.
外泌体携带具有重大生物学和临床意义的分子，如微小RNA（miRNA）。外泌体的内容物在健康对照者和癌症患者之间存在差异。因此，外泌体中运输的miRNA和其他分子被认为是癌症诊断和预后生物标志物的潜在来源。近年来已检测到许多miRNA。因此，有大量比较患者和健康个体的miRNA相关数据，这有助于使用非侵入性采样程序更好地理解癌症的发生、发展、恶性程度和转移。然而，对现有非编码RNA（ncRNA）数据进行重新分析的情况很少见。本研究利用外泌体中miRNA的现有数据，比较健康个体和癌症患者，以确定与癌症存在相关的可能的整体变化。一项强有力的转录组分析确定了在五个癌症数据集中失调的两种常见miRNA（miR-495-3p和miR-543）。它们已被发现与不同癌症有关，但在血液中循环的外泌体中尚未见报道。该研究还检查了它们的靶基因以及这些基因对功能过程的影响。
