Department of Thyroid and Breast Surgery, Affiliated Hospital of Jianghan University, Wuhan, 430000, Hubei, China.
Department of Thyroid and Breast Surgery, Affiliated Hospital of Jianghan University, Wuhan, 430000, Hubei, China.
Biochem Biophys Res Commun. 2021 Sep 17;570:191-198. doi: 10.1016/j.bbrc.2021.07.005. Epub 2021 Jul 19.
Breast cancer (BC) continues to plague millions of people worldwide. MicroRNAs have been observed to be closely associated with many cancers and may serve as promising biomarkers for the diagnosis of BC. BC tissue samples were collected from 26 patients, and qRT-PCR and western blotting were performed to evaluate the levels of miR-543 and VCAN. The action of miR-543 and VCAN was determined using CCK-8, BrdU, wound healing, and transwell invasion assays. Luciferase and RNA pull-down assays were used to assess whether miR-543 bound to VCAN. We found that miR-543 inhibited BC cell viability, proliferation, migration, and invasion by repressing the expression of VCAN. VCAN was upregulated in BC tissues and exerted beneficial effects on the development process of BC. Our results highlighted that the miR-543/VCAN axis is a promising diagnostic and prognostic biomarker in clinical applications.
乳腺癌（BC）仍然困扰着全世界数百万人。已经观察到 microRNAs 与许多癌症密切相关，并且可能作为 BC 诊断的有前途的生物标志物。从 26 名患者中收集了 BC 组织样本，并进行了 qRT-PCR 和 Western blot 以评估 miR-543 和 VCAN 的水平。使用 CCK-8、BrdU、划痕愈合和 Transwell 侵袭测定来确定 miR-543 和 VCAN 的作用。荧光素酶和 RNA 下拉测定用于评估 miR-543 是否与 VCAN 结合。我们发现 miR-543 通过抑制 VCAN 的表达来抑制 BC 细胞的活力、增殖、迁移和侵袭。VCAN 在 BC 组织中上调，并对 BC 的发展过程发挥有益作用。我们的结果强调，miR-543/VCAN 轴是临床应用中有前途的诊断和预后生物标志物。
