Department of Biology Education, Faculty of Education, Universitas Bung Hatta, Padang, West Sumatera, 25133, Indonesia.
Department of Aquaculture, Faculty of Fisheries and Marine Science, Universitas Bung Hatta, Padang, West Sumatera, 25133, Indonesia.
F1000Res. 2021 Sep 15;10:922. doi: 10.12688/f1000research.53760.2. eCollection 2021.
The giant gourami sago strain ( Lacepède) has been approved in 2018 as a candidate for freshwater aquaculture in Indonesia. However, information on the species' reproduction is minimal. This study analyzed the reproductive characteristics of the gourami sago strain broodfish to provide basic knowledge for a future hatchery development strategy. A total of 10 female and 10 male mature gourami sago strain broodfish were measured for body weight and length, and were evaluated for their reproductive characteristics. Breeding fish were spawned naturally in a 2×1×0.6 m concrete pond with a male-female sex ratio of 1:1. Egg weight and diameter were measured in 25 eggs per female using, respectively, ACIS AD- 600i scales with 0.01 g accuracy, and a microscope (Labo model L-711) using Canon Digital Camera Software 3 . Semen was collected using plastic syringes in 3 mL aliquots, then placed in an insulated ice-cooled container, and analyzed within two hours of collection. Average weights of female and male broodfish before spawning were 2180±159.78 g and 3060±134.99 g, respectively. The relative fecundity and egg diameter were 1029±36 eggs kg and 2.42±0.05 mm, respectively. The hatching rate and embryo survival to an eyed-egg stage were respectively 76.40±2.27% and 94.76±0.42%. Sperm characteristics showed that volume was 0.60±0.12 ml kg and percentage of motile sperm was 70.04±2.27%. Female broodfish weight after spawning was strongly correlated with the weight before spawned ( = 0.999) and absolute fecundity was also strongly correlated with female broodfish weight before spawning ( = 0.921). Sperm concentration was moderately correlated with sperm motility ( = 0.556) and duration of sperm motility ( = 0.502). The gourami sago strain broodfish has suitable reproductive characteristics for the development of hatcheries. Successful natural spawning should be followed by larval weaning and feeding technology to increase growth and survival.
巨型丝足鲈萨格氏亚种（ Lacepède）于 2018 年被印度尼西亚批准作为淡水水产养殖的候选品种。然而，有关该物种繁殖的信息却很少。本研究分析了丝足鲈萨格氏亚种亲鱼的繁殖特征，为未来的孵化场发展战略提供了基础知识。
总共测量了 10 条成熟的雌性丝足鲈萨格氏亚种亲鱼和 10 条成熟的雄性丝足鲈萨格氏亚种亲鱼的体重和体长，并评估了它们的繁殖特征。繁殖鱼在一个 2×1×0.6 m 的混凝土池塘中自然产卵，雌雄比例为 1:1。使用精度为 0.01 g 的 ACIS AD-600i 秤和佳能数码相机关联软件 3 ，分别测量了每尾雌鱼的 25 枚卵的卵重和卵径。精液用塑料注射器采集 3 mL 等分，然后放入隔热冰冷却容器中，在采集后两小时内进行分析。产卵前雌性和雄性亲鱼的平均体重分别为 2180±159.78 g 和 3060±134.99 g。相对繁殖力和卵径分别为 1029±36 个卵·kg 和 2.42±0.05 mm。孵化率和胚胎发育至眼点期的存活率分别为 76.40±2.27%和 94.76±0.42%。精液特征表明，体积为 0.60±0.12 ml·kg ，运动精子的比例为 70.04±2.27%。产卵后雌鱼体重与产卵前体重呈强正相关（ = 0.999），绝对繁殖力也与产卵前雌鱼体重呈强正相关（ = 0.921）。精子浓度与精子活力（ = 0.556）和精子活力持续时间（ = 0.502）呈中度相关。丝足鲈萨格氏亚种亲鱼具有适合孵化场发展的繁殖特征。成功的自然产卵后，应进行幼鱼离乳和饲养技术，以提高生长和成活率。
