Department of Central Laboratory, Department of Laboratory Medicine, the Second People's Hospital of Lianyungang City (Cancer Hospital of Lianyungang), Lianyungang, China.
School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, China.
Front Cell Infect Microbiol. 2022 May 11;12:876552. doi: 10.3389/fcimb.2022.876552. eCollection 2022.
is a worldwide, primary cause of respiratory tract infections, septicemia, urinary apparatus infections, and secondary meningitis. It can be fatal. Rapid and accurate detection methods are needed to control the spread of carbapenem-resistant (CRAB). Current molecular diagnostic methods are limited and not suitable for on-site detection. In this study, an isothermal detection method using recombinase polymerase amplification (RPA) combined with a lateral flow strip (LFS) was developed to target the and genes of . . The reaction was completed in about 40 min at 37°C. This method can also effectively distinguish and CRAB. The limit of detection of 10-10 CFU/reaction was equal to that of other detection methods. The detection accuracy was equal to that of the qPCR method with the use of clinical samples. The RPA-LFS assay is portable, rapid, and accurate and could replace existing detection methods for on-site detection of . and CRAB.
是一种全球性的呼吸道感染、败血症、尿路感染和继发性脑膜炎的主要病原体。它可能是致命的。需要快速准确的检测方法来控制碳青霉烯类耐药菌（CRAB）的传播。目前的分子诊断方法有限，不适合现场检测。在本研究中，开发了一种使用重组酶聚合扩增（RPA）与侧流条（LFS）相结合的等温检测方法，以针对 和 基因。反应在 37°C 下约 40 分钟完成。该方法还可以有效区分 和 CRAB。10-10 CFU/反应的检测限与其他检测方法相当。使用临床样本时，检测准确性与 qPCR 方法相当。RPA-LFS 检测法便携、快速、准确，可以替代现有的现场检测方法，用于检测 和 CRAB。
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