Ranjbar Reza, Zayeri Shahin, Mirzaie Amir
Molecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Iran J Microbiol. 2020 Apr;12(2):107-112.
has been known as a major pathogen causing nosocomial infections. The aim of this study was to develop multiplex PCR for rapid and simultaneous detection of metallo-β-lactamase (MBL) genes in clinical isolates of .
In this study, we used three sets of primers to amplify the MBL genes including , and . The multiplex PCR assay was optimized for rapid and simultaneous detection of MBL genes in strains recovered from clinical samples.
strains recovered from clinical samples were subjected to the study. The multiplex PCR produced 3 bands of 501 bp for , 744 bp for and 623 bp for genes. In addition to, no any cross-reactivity was observed in multiplex PCR.
Based on obtained data, the multiplex PCR had a good specificity without any cross reactivity and it appears that the multiplex PCR is reliable assay for simultaneous detection of MBL genes in strains.
已知是引起医院感染的主要病原体。本研究的目的是开发多重聚合酶链反应（PCR），用于快速同时检测临床分离株中的金属β-内酰胺酶（MBL）基因。
在本研究中，我们使用了三组引物来扩增MBL基因，包括、和。对多重PCR检测方法进行了优化，以快速同时检测从临床样本中分离出的菌株中的MBL基因。
对从临床样本中分离出的菌株进行了研究。多重PCR产生了三条带，基因的条带为501 bp，基因的条带为744 bp，基因的条带为623 bp。此外，在多重PCR中未观察到任何交叉反应。
根据获得的数据，多重PCR具有良好的特异性，无任何交叉反应，似乎多重PCR是同时检测菌株中MBL基因的可靠检测方法。
