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甲状腺激素缺乏会损害雄性生殖细胞发育：斑马鱼下丘脑 - 垂体 - 甲状腺轴与性腺轴之间的相互作用

Thyroid Hormones Deficiency Impairs Male Germ Cell Development: A Cross Talk Between Hypothalamic-Pituitary-Thyroid, and-Gonadal Axes in Zebrafish.

作者信息

Rodrigues Maira S, Tovo-Neto Aldo, Rosa Ivana F, Doretto Lucas B, Fallah Hamideh P, Habibi Hamid R, Nóbrega Rafael H

机构信息

Aquaculture Program (CAUNESP), São Paulo State University (UNESP), São Paulo, Brazil.

Reproductive and Molecular Biology Group, Department of Structural and Functional Biology, Institute of Biosciences, São Paulo State University (UNESP), Botucatu, Brazil.

出版信息

Front Cell Dev Biol. 2022 May 12;10:865948. doi: 10.3389/fcell.2022.865948. eCollection 2022.

DOI:10.3389/fcell.2022.865948
PMID:35646887
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9133415/
Abstract

In vertebrates, thyroid hormones are critical players in controlling different physiological processes such as development, growth, metabolism among others. There is evidence in mammals that thyroid hormones are also an important component of the hormonal system that controls reproduction, although studies in fish remain poorly investigated. Here, we tested this hypothesis by investigating the effects of methimazole-induced hypothyroidism on the testicular function in adult zebrafish. Treatment of fish with methimazole, , significantly altered zebrafish spermatogenesis by inhibiting cell differentiation and meiosis, as well as decreasing the relative number of spermatozoa. The observed impairment of spermatogenesis by methimazole was correlated with significant changes in transcript levels for several genes implicated in the control of reproduction. Using an approach, we also demonstrated that in addition to affecting the components of the brain-pituitary-peripheral axis, T3 (triiodothyronine) also exerts direct action on the testis. These results reinforce the hypothesis that thyroid hormones are an essential element of multifactorial control of reproduction and testicular function in zebrafish and possibly other vertebrate species.

摘要

在脊椎动物中，甲状腺激素在控制不同生理过程（如发育、生长、新陈代谢等）中起着关键作用。在哺乳动物中有证据表明，甲状腺激素也是控制生殖的激素系统的重要组成部分，尽管鱼类方面的研究仍较少。在此，我们通过研究甲巯咪唑诱导的甲状腺功能减退对成年斑马鱼睾丸功能的影响来验证这一假设。用甲巯咪唑处理鱼类，显著改变了斑马鱼的精子发生过程，抑制了细胞分化和减数分裂，同时减少了精子的相对数量。观察到的甲巯咪唑对精子发生的损害与几个参与生殖控制的基因转录水平的显著变化相关。使用一种方法，我们还证明，除了影响脑 - 垂体 - 外周轴的组成部分外，三碘甲状腺原氨酸（T3）也对睾丸发挥直接作用。这些结果强化了以下假设：甲状腺激素是斑马鱼以及可能其他脊椎动物物种生殖和睾丸功能多因素控制的基本要素。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0c0/9133415/a66ba8a45b29/fcell-10-865948-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0c0/9133415/d246a8b9fbe6/fcell-10-865948-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0c0/9133415/0ae9b2a16a88/fcell-10-865948-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0c0/9133415/42f3eb4dacb3/fcell-10-865948-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0c0/9133415/2c813c8f7d37/fcell-10-865948-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0c0/9133415/e7e88a8293b6/fcell-10-865948-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0c0/9133415/a66ba8a45b29/fcell-10-865948-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0c0/9133415/d246a8b9fbe6/fcell-10-865948-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0c0/9133415/0ae9b2a16a88/fcell-10-865948-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0c0/9133415/42f3eb4dacb3/fcell-10-865948-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0c0/9133415/2c813c8f7d37/fcell-10-865948-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0c0/9133415/e7e88a8293b6/fcell-10-865948-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d0c0/9133415/a66ba8a45b29/fcell-10-865948-g006.jpg

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3
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