Cho Whirang, Rafi Harmain, Cho Seulki, Balijepalli Arvind, Zestos Alexander G
Department of Chemistry, American University, Washington, D.C. 20016, USA.
Biophysical and Biomedical Measurement Group, Microsystems and Nanotechnology Division, National Institute of Standards and Technology, Gaithersburg 20899, USA.
Sens Diagn. 2022 May 1;1(3):460-464. doi: 10.1039/d2sd00023g. Epub 2022 Apr 5.
Rapid and sensitive pH measurements with increased spatiotemporal resolution are imperative to probe neurochemical signals and illuminate brain function. We interfaced carbon fiber microelectrode (CFME) sensors with both fast scan cyclic voltammetry (FSCV) and field-effect transistor (FET) transducers for dynamic pH measurements. The electrochemical oxidation and reduction of functional groups on the surface of CFMEs affect their response over a physiologically relevant pH range. When measured with FET transducers, the sensitivity of the measurements over the measured pH range was found to be (101 ± 18) mV, which exceeded the Nernstian value of 59 mV by approximately 70%. Finally, we validated the functionality of CFMEs as pH sensors with FSCV in rat brain coronal slices with exogenously applied solutions of varying pH values indicating that potential study is feasible.
为了探测神经化学信号并阐明脑功能，快速且灵敏的pH测量以及提高时空分辨率至关重要。我们将碳纤维微电极（CFME）传感器与快速扫描循环伏安法（FSCV）和场效应晶体管（FET）换能器相结合，用于动态pH测量。CFME表面官能团的电化学氧化和还原会影响其在生理相关pH范围内的响应。当使用FET换能器进行测量时，发现在测量的pH范围内测量灵敏度为（101±18）mV，比能斯特值59 mV高出约70%。最后，我们通过在大鼠脑冠状切片中使用不同pH值的外源溶液验证了CFME作为pH传感器与FSCV的功能，表明潜在的研究是可行的。
