Department of Biomedical Engineering, The Catholic University of America, Washington, D.C., 20064, USA.
Department of Chemistry, American University, Washington, D.C., 20016, USA.
Anal Bioanal Chem. 2024 Sep;416(21):4807-4818. doi: 10.1007/s00216-024-05373-y. Epub 2024 Jun 25.
The hormone Neuropeptide Y (NPY) plays critical roles in feeding, satiety, obesity, and weight control. However, its complex peptide structure has hindered the development of fast and biocompatible detection methods. Previous studies utilizing electrochemical techniques with carbon fiber microelectrodes (CFMEs) have targeted the oxidation of amino acid residues like tyrosine to measure peptides. Here, we employ the modified sawhorse waveform (MSW) to enable voltammetric identification of NPY through tyrosine oxidation. Use of MSW improves NPY detection sensitivity and selectivity by reducing interference from catecholamines like dopamine, serotonin, and others compared to the traditional triangle waveform. The technique utilizes a holding potential of -0.2 V and a switching potential of 1.2 V that effectively etches and renews the CFME surface to simultaneously detect NPY and other monoamines with a sensitivity of 5.8 ± 0.94 nA/µM (n = 5). Furthermore, we observed adsorption-controlled, subsecond NPY measurements with CFMEs and MSW. The effective identification of exogenously applied NPY in biological fluids demonstrates the feasibility of this methodology for in vivo and ex vivo studies. These results highlight the potential of MSW voltammetry to enable fast, biocompatible NPY quantification to further elucidate its physiological roles.
神经肽 Y（NPY）是一种激素，在进食、饱腹感、肥胖和体重控制中起着关键作用。然而，其复杂的肽结构阻碍了快速和生物相容的检测方法的发展。以前利用碳纤维微电极（CFME）的电化学技术的研究针对酪氨酸等氨基酸残基的氧化来测量肽。在这里，我们采用改良的锯形波（MSW）通过酪氨酸氧化来实现 NPY 的伏安识别。与传统的三角波相比，MSW 的使用通过减少儿茶酚胺（如多巴胺、血清素和其他物质）的干扰，提高了 NPY 检测的灵敏度和选择性。该技术使用-0.2 V 的保持电位和 1.2 V 的切换电位，有效地蚀刻和更新 CFME 表面，同时以 5.8±0.94 nA/µM（n = 5）的灵敏度检测 NPY 和其他单胺。此外，我们观察到 CFME 和 MSW 对吸附控制的亚秒级 NPY 测量。在外源性 NPY 在生物流体中的有效识别证明了这种方法在体内和体外研究中的可行性。这些结果突出了 MSW 伏安法在快速、生物相容的 NPY 定量中的潜力，以进一步阐明其生理作用。
