Key Laboratory for Advanced Materials, Joint International Laboratory for Precision Chemistry & School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai, 200237, PR China.
Department of Rheumatology and Immunology, Shanghai Jiao Tong University Medical School Affiliated Ruijin Hospital, Shanghai, 200020, PR China.
Talanta. 2022 Sep 1;247:123620. doi: 10.1016/j.talanta.2022.123620. Epub 2022 May 27.
There is an unmet clinical need to develop noninvasive liquid biopsy tools for systemic lupus erythematosus (SLE) diagnosis and therapeutic effect evaluation. Extracellular vesicles (EVs), which are abundant in body fluids, have emerged as a valuable resource for liquid biopsy. Herein, we describe a simple and robust EV detection platform that is based on a plasmonic nanoparticle-embedded polydopamine substrate that is modified with EV-capture molecules and detection probes. We investigated three EV biomarkers, namely, programmed cell death protein-1 (PD-1), microRNA-146a (miRNA-146a) and sialic acid (SA), in serum and urine from SLE patients and healthy controls. This platform prevents complex pretreatment while enabling highly efficient EV capture to the substrate surface, and the multiple functionalization of the detection interface with specific biomarker probes enables simultaneous detection of PD-1, miRNA-146a and SA that are carried by EVs via fluorescence (FL) imaging at the single-vesicle level. Via comparison of EV biomarker profiles, SLE patients can be distinguished from normal controls and classified into treated and untreated groups. Due to its ease of preparation, simplicity and stability, our approach shows good potential in the design of EV-based biosensors for clinical use.
临床上需要开发非侵入性的液体活检工具来诊断系统性红斑狼疮（SLE）并评估治疗效果。细胞外囊泡（EVs）在体液中大量存在，已成为液体活检的有价值资源。本文描述了一种基于等离子体纳米粒子嵌入聚多巴胺基底的简单而稳健的 EV 检测平台，该平台用 EV 捕获分子和检测探针进行了修饰。我们研究了三种 EV 生物标志物，即程序性死亡蛋白-1（PD-1）、微小 RNA-146a（miRNA-146a）和唾液酸（SA），在 SLE 患者和健康对照者的血清和尿液中。该平台可防止复杂的预处理，同时实现对基底表面的高效 EV 捕获，并且通过特定生物标志物探针对检测界面进行多功能化，可通过荧光（FL）成像在单囊泡水平上同时检测 EV 携带的 PD-1、miRNA-146a 和 SA。通过比较 EV 生物标志物谱，可以将 SLE 患者与正常对照者区分开来，并将其分为治疗组和未治疗组。由于其制备简单、操作简便、稳定性好，该方法在基于 EV 的生物传感器的临床应用设计中具有良好的应用潜力。
