Valero-Guillén P, Martin-Luengo F, Jimenez J, Larsson L
Acta Pathol Microbiol Immunol Scand B. 1986 Dec;94(6):373-6. doi: 10.1111/j.1699-0463.1986.tb03070.x.
A two-dimensional thin-layer chromatographic method was developed which allowed separation of the different mycobacterial mycolic acids as methyl esters. Dichloromethane (once) and petroleum ether:acetone (95:5, v/v twice or three times) were used as solvents. Alkaline saponification of freeze-dried cells followed by methylation of the mycolic acids using iodomethane gave satisfactory results, whereas methylation using boron trichloride-methanol complex or trans-esterification through direct acid methanolysis was found to degrade epoxy-mycolates. The chromatographic method developed here is rapid and informative, and should prove valuable in routine mycobacterial differentiation.
开发了一种二维薄层色谱法，可将不同的分枝杆菌霉菌酸分离为甲酯。使用二氯甲烷（一次）和石油醚：丙酮（95:5，v/v，两次或三次）作为溶剂。冻干细胞的碱性皂化，然后使用碘甲烷对霉菌酸进行甲基化，得到了满意的结果，而使用三氯化硼 - 甲醇络合物进行甲基化或通过直接酸性甲醇解进行酯交换反应会导致环氧霉菌酸降解。此处开发的色谱方法快速且信息丰富，在分枝杆菌的常规鉴别中应具有重要价值。
