Genetics and Biochemistry Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, USA; email:
Current affiliation: School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, New South Wales, Australia; email:
Annu Rev Microbiol. 2022 Sep 8;76:259-279. doi: 10.1146/annurev-micro-033021-023719. Epub 2022 Jun 1.
The Omp85 protein superfamily is found in the outer membrane (OM) of all gram-negative bacteria and eukaryotic organelles of bacterial origin. Members of the family catalyze both the membrane insertion of β-barrel proteins and the translocation of proteins across the OM. Although the mechanism(s) by which these proteins function is unclear, striking new insights have emerged from recent biochemical and structural studies. In this review we discuss the entire Omp85 superfamily but focus on the function of the best-studied member, BamA, which is an essential and highly conserved component of the bacterial barrel assembly machinery (BAM). Because BamA has multiple functions that overlap with those of other Omp85 proteins, it is likely the prototypical member of the Omp85 superfamily. Furthermore, BamA has become a protein of great interest because of the recent discovery of small-molecule inhibitors that potentially represent an important new class of antibiotics.
Omp85 蛋白超家族存在于所有革兰氏阴性菌的外膜（OM）和具有细菌起源的真核细胞器中。该家族的成员可催化 β-桶状蛋白的膜插入和蛋白质穿过 OM 的易位。尽管这些蛋白质的作用机制尚不清楚，但最近的生化和结构研究揭示了一些引人注目的新见解。在这篇综述中，我们讨论了整个 Omp85 超家族，但重点介绍了研究最充分的成员 BamA 的功能，BamA 是细菌桶状组装机制（BAM）的必需且高度保守的组成部分。由于 BamA 具有与其他 Omp85 蛋白重叠的多种功能，因此它很可能是 Omp85 超家族的典型成员。此外，由于最近发现了可能代表一类重要新型抗生素的小分子抑制剂，BamA 已成为一种备受关注的蛋白质。
