Truong Vinh X, Li Fanyi, Forsythe John S
Department of Materials Science and Engineering, Monash Institute of Medical Engineering, Monash University, Clayton, 3800 VIC, Australia.
CSIRO Manufacturing, Bayview Avenue, Clayton, VIC 3168, Australia.
ACS Macro Lett. 2017 Jul 18;6(7):657-662. doi: 10.1021/acsmacrolett.7b00312. Epub 2017 Jun 13.
Recent developments in photochemistry have introduced new methods to prepare hydrogels initiated by nonharmful light which is essential for encapsulation of cells and bioactive components. However, bioorthogonal photoclick reactions generally requires two components for cross-linking and, in many cases, the formation of a reactive intermediate that may cross-react with nucleophiles in biological media. Here we report the utilization of a visible light triggered dimerization of electron-rich anthracene for polymer cross-linking to form bulk hydrogels and microgels. Incorporation of gelatin within the hydrogel enhanced cell attachment and viability after 7 days of culture and spatiotemporal conjugation of a bioactive component using photochemical dimerization of anthracene was demonstrated. This work therefore introduces a simple yet powerful tool for light modulated bioorthogonal polymer cross-linking, which can be utilized in various bioengineering applications.
光化学领域的最新进展引入了新方法，可制备由无害光引发的水凝胶，这对于细胞和生物活性成分的封装至关重要。然而，生物正交光点击反应通常需要两种组分进行交联，并且在许多情况下，会形成可能与生物介质中的亲核试剂发生交叉反应的反应性中间体。在此，我们报道利用富电子蒽的可见光触发二聚作用进行聚合物交联，以形成块状水凝胶和微凝胶。在水凝胶中加入明胶可增强培养7天后的细胞附着和活力，并证明了使用蒽的光化学二聚作用对生物活性成分进行时空共轭。因此，这项工作引入了一种简单而强大的光调制生物正交聚合物交联工具，可用于各种生物工程应用。
