Faculty of Food Science and Engineering, Kunming University of Science and Technology, Kunming 650500, China.
Department of Cardiology, The First People's Hospital of Yunnan, Kunming 650000, China.
Food Res Int. 2022 Jun;156:111196. doi: 10.1016/j.foodres.2022.111196. Epub 2022 Mar 28.
In this study, the protective effects of hot water (QW) and aqueous-ethanol extracts (QA) from Que Zui tea on non-alcoholic fatty liver disease (NAFLD) were investigated. Quantitative and qualitative analysis revealed that QW and QA were rich in polyphenols, especially 6'-O-caffeoylarbutin. Both QW and QA significantly reduced body weight and liver index, increased serum levels of high density lipoprotein cholesterol (HDL-C), and decreased the levels of total cholesterol (TC), triglyceride (TG), nonesterified free fatty acids (NEFA) and low density lipoprotein cholesterol (LDL-C) in NAFLD rats induced high fat diet. Furthermore, the contents of TC, TG, NEFA, alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the liver tissues were inhibited after QW and QA administration. Histopathological examination showed that QW and QA significantly reduced liver lipid accumulation of NAFLD rats. In addition, QW and QA could enhance increase the activity of antioxidant (glutathione, superoxide dismutase and catalase) in the liver by regulation Nrf2 signaling pathway, thereby alleviating liver damage caused by lipid peroxidation. QW and QA activated AMPK/PPAR-α signaling pathway by increasing the expression of adiponectin and its receptor AdipoR2, thereby reducing fat production and enhancing fatty acid β oxidation. These data suggested that QW and QA had the potential to in the prevention and treatment of NAFLD.
本研究探讨了热水（QW）和 Que Zui 茶的水醇提取物（QA）对非酒精性脂肪肝病（NAFLD）的保护作用。定量和定性分析表明，QW 和 QA 富含多酚，尤其是 6'-O-咖啡酰基阿拉伯糖苷。QW 和 QA 均能显著降低 NAFLD 大鼠的体重和肝指数，增加血清高密度脂蛋白胆固醇（HDL-C）水平，降低总胆固醇（TC）、甘油三酯（TG）、非酯化游离脂肪酸（NEFA）和低密度脂蛋白胆固醇（LDL-C）水平。此外，QW 和 QA 给药后可抑制肝组织中 TC、TG、NEFA、丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）的含量。组织病理学检查表明，QW 和 QA 可显著减少 NAFLD 大鼠肝脏的脂质蓄积。此外，QW 和 QA 可通过调节 Nrf2 信号通路增强肝脏抗氧化剂（谷胱甘肽、超氧化物歧化酶和过氧化氢酶）的活性，从而减轻脂质过氧化引起的肝损伤。QW 和 QA 通过增加脂联素及其受体 AdipoR2 的表达，激活 AMPK/PPAR-α 信号通路，从而减少脂肪生成并增强脂肪酸β氧化。这些数据表明，QW 和 QA 具有预防和治疗 NAFLD 的潜力。
