Hinckley Joshua A, Chapman Dana V, Hedderick Konrad R, Oleske Katharine W, Estroff Lara A, Wiesner Ulrich B
ACS Macro Lett. 2019 Oct 15;8(10):1378-1382. doi: 10.1021/acsmacrolett.9b00675. Epub 2019 Oct 2.
In recent years, high-resolution optical imaging in the far field has provided opportunities for alternative approaches to nanocharacterization traditionally dominated by electron and scanning probe microscopies. Here, we report the optical super-resolution imaging of model block copolymer (BCP) thin film surface nanostructures through stochastic optical reconstruction microscopy (STORM). We compare a set of surface-functionalized fluorescent core-shell silica nanoparticles encapsulating two different organic dyes, Cy3 and Cy5, with the corresponding free dyes in STORM. Using various click-type chemistries, these probes are covalently attached to the surface of specific blocks of BCP thin films, enabling selective block labeling and optical visualization. We demonstrate that the enhanced brightness of these particle probes offers distinct advantages over conventional dye labeling, outperforming one of the best STORM dyes available (Cy5).
近年来，远场高分辨率光学成像为传统上由电子显微镜和扫描探针显微镜主导的纳米表征提供了替代方法。在此，我们报告了通过随机光学重建显微镜（STORM）对模型嵌段共聚物（BCP）薄膜表面纳米结构进行的光学超分辨率成像。我们在STORM中比较了一组封装两种不同有机染料（Cy3和Cy5）的表面功能化荧光核壳二氧化硅纳米颗粒与相应的游离染料。利用各种点击型化学方法，这些探针共价连接到BCP薄膜特定嵌段的表面，实现选择性嵌段标记和光学可视化。我们证明，这些颗粒探针增强的亮度相对于传统染料标记具有明显优势，优于现有的最佳STORM染料之一（Cy5）。
