Manan Hidayah, Amin-Safwan Adnan, Shakri Irma Wahida, Kasan Nor Azman, Ikhwanuddin Mhd
Higher Institution Centre of Excellent (HICoE), Institute of Tropical Aquaculture and Fisheries, Universiti Malaysia Terengganu, 21030 Kuala Nerus, Terengganu, Malaysia.
Trop Life Sci Res. 2022 Mar;33(1):91-103. doi: 10.21315/tlsr2022.33.1.6. Epub 2022 Mar 31.
Study on ovarian maturation stages were carried out using external morphological assessment of ovarian colourations and histological assessment of Oocyte structure for broodstock selection purpose. Stage 1 to Stage 4 of female shrimps broodstock were sampled from Kuala Muda, Kedah, Malaysia. Four obvious colourations of ovary stages were identified which are: yellow (Stage 1), green yellowish (Stage 2), light greenish (Stage 3) and dark green (Stage 4) through the morphological assessment. The Gonadosomatic Index (GSI) showed significant increment as the gonad reach advance stages. Histological assessment of Stage 1 ovary identified perinucleolar oocyte (PO) with small size oocytes. Stage 2 ovary was identified with yolkless oocyte (YO), Stage 3 ovary was identified with late stage of yolky globules and Stage 4 ovary was identified with fully yolky globules. From the results achieved, it can be concluded that the ovarian colourations and GSI can be applied to identify the ovarian maturation stages, meanwhile, the histology assessment is the most precise method to determine the ovarian maturation stages in more details and accurate for each of the ovarian maturation stages.
为了进行亲虾选择，通过对卵巢颜色的外部形态评估和卵母细胞结构的组织学评估，开展了卵巢成熟阶段的研究。从马来西亚吉打州瓜拉慕达采集了雌虾亲体的1期至4期样本。通过形态学评估，确定了卵巢阶段的四种明显颜色，即：黄色（1期）、黄绿色（2期）、浅绿（3期）和深绿（4期）。随着性腺发育到后期，性腺指数（GSI）显著增加。对1期卵巢的组织学评估发现了小尺寸卵母细胞的核仁周围卵母细胞（PO）。2期卵巢鉴定为无卵黄卵母细胞（YO），3期卵巢鉴定为卵黄球后期，4期卵巢鉴定为完全充满卵黄球。根据所取得的结果可以得出结论，卵巢颜色和GSI可用于识别卵巢成熟阶段，同时，组织学评估是更详细、准确地确定每个卵巢成熟阶段卵巢成熟阶段的最精确方法。
