Saputra Ishaaq, Lee Yih Nin, Fotedar Ravi
Faculty of Engineering and Sciences Curtin University, CDT 250, Miri Sarawak 98009, Malaysia.
School of Molecular and Life Sciences Curtin University, Kent Street, Bentley 6102, WA, Australia.
Aquac Nutr. 2024 Jun 25;2024:8579991. doi: 10.1155/2024/8579991. eCollection 2024.
The present study aims to evaluate the effect of liquid fish protein hydrolysate (FPH) following fishmeal substitution with full-fat and defatted BSF (black soldier fly, ) meal in the feeds of juvenile ornate spiny lobster, . The physiological aspects of juvenile lobsters including growth, fatty acids profile, and histopathology were observed. Six isoenergetic experimental feeds having a protein-to-energy ratio of 26 CP mg kJ were formulated with the substitution of fishmeal at 25% using liquid FPH, full-fat BSF (FBSF), defatted BSF (DBSF), and their combination. The specific growth rate, final body weight, final total length, and length increment of juvenile lobsters (initial weight was 0.21 ± 0.01 g and total length was 20.53 ± 0.12 mm) were significantly affected by the fishmeal substitution ( < 0.05) and improved with the addition of liquid FPH in the feeds containing FBSF and DBSF. The whole body proximate analysis showed that the liquid FPH to the feeds containing DBSF increased the ash and protein content significantly ( < 0.05). The total monounsaturated fatty acids (∑MUFA), saturated fatty acids (∑SFA), and omega 9 fatty acids (∑-9 FA) of juvenile lobsters' whole bodies fed with dietary DBSF and FPH supplementation were significantly higher than those of others ( < 0.05). The histopathological analysis indicated that the villus size and the muscle thickness in the intestine were not significantly affected by FPH supplementation. However, the hepatopancreas histopathology indicated the presence of B-cells and R-cells in the juvenile lobsters fed with FPH-supplemented feeds. The present results suggested the supplementation of liquid FPH to the formulated feed with FBSF and DBSF for juvenile lobsters can improve the lobsters' growth and fatty acids availability.
本研究旨在评估用全脂和脱脂黑水虻（BSF）粉替代鱼粉后，液态鱼蛋白水解物（FPH）对华丽刺龙虾幼体饲料的影响。观察了幼体龙虾的生理方面，包括生长、脂肪酸谱和组织病理学。配制了六种等能实验饲料，蛋白质与能量比为26 CP mg kJ，使用液态FPH、全脂黑水虻（FBSF）、脱脂黑水虻（DBSF）及其组合以25%的比例替代鱼粉。鱼粉替代对幼体龙虾（初始体重为0.21 ± 0.01 g，全长为20.53 ± 0.12 mm）的特定生长率、终末体重、终末全长和体长增加有显著影响（ < 0.05），并且在含有FBSF和DBSF的饲料中添加液态FPH后有所改善。全身体成分分析表明，向含有DBSF的饲料中添加液态FPH显著增加了灰分和蛋白质含量（ < 0.05）。喂食添加了DBSF和FPH的饲料的幼体龙虾全身的总单不饱和脂肪酸（∑MUFA）、饱和脂肪酸（∑SFA）和ω-9脂肪酸（∑-9 FA）显著高于其他组（ < 0.05）。组织病理学分析表明，补充FPH对肠绒毛大小和肌肉厚度没有显著影响。然而，肝胰腺组织病理学表明，喂食补充了FPH的饲料的幼体龙虾中存在B细胞和R细胞。目前的结果表明，在为华丽刺龙虾幼体配制的饲料中添加液态FPH可以改善龙虾的生长和脂肪酸利用率。
