Husak Viktor, Strutynska Tetiana, Burdyliuk Nadia, Pitukh Anzhelika, Bubalo Volodymyr, Falfushynska Halina, Strilbytska Olha, Lushchak Oleh
Department of Biochemistry and Biotechnology, Vasyl Stefanyk Precarpathian National University, 57 Shevchenka Str., Ivano-Frankivsk, 76018, Ukraine.
Laboratory of Experimental Toxicology and Mutagenesis, L.I. Medved's Research Center of Preventive Toxicology, Food and Chemical Safety, MHU, Kyiv, Ukraine.
EXCLI J. 2022 Mar 9;21:595-609. doi: 10.17179/excli2022-4690. eCollection 2022.
The use of glyphosate-based Roundup and triazine herbicide Atrazine has increased markedly in last decades. Thus, it is important to evaluate toxic effects of these herbicides to non-targeted organisms such as zooplankton to understand their safety toward aquatic ecosystems. In the current study, we performed Daphnia toxicity tests based on lethality to identify LC that provides acute aquatic toxicity classification criteria. LC for Roundup exposure for 24 hours was found to be 0.022 mg/L and 48 hours - 0.0008 mg/L. Atrazine showed LC at concentrations of 40 mg/L and 7 mg/L for 24 and 48 hours, respectively. We demonstrated that exposure to ecologically relevant concentrations of Roundup or Atrazine decreases lipid peroxidation and protein thiol levels, however caused increase in carbonyl protein and low-molecular-weight thiols content. Moreover, the herbicide treatments caused increase of superoxide dismutase activity. Our data suggest that at very low concentrations Roundup and Atrazine disturb free radical processes in .
在过去几十年中，基于草甘膦的农达和三嗪类除草剂阿特拉津的使用显著增加。因此，评估这些除草剂对浮游动物等非目标生物的毒性作用，对于了解它们对水生生态系统的安全性至关重要。在当前的研究中，我们基于致死率进行了水蚤毒性测试，以确定提供急性水生毒性分类标准的半数致死浓度（LC）。发现农达暴露24小时的半数致死浓度为0.022毫克/升，48小时为0.0008毫克/升。阿特拉津在24小时和48小时的半数致死浓度分别为40毫克/升和7毫克/升。我们证明，暴露于生态相关浓度的农达或阿特拉津会降低脂质过氧化和蛋白质硫醇水平，但会导致羰基蛋白和低分子量硫醇含量增加。此外，除草剂处理导致超氧化物歧化酶活性增加。我们的数据表明，在非常低的浓度下，农达和阿特拉津会干扰自由基过程。
