Sun Wei, Sun Shiyu, Xu Hui, Wang Yuhan, Chen Yiran, Xu Xiaorong, Yi Yin, Ju Zhigang
Key Laboratory of State Forestry Administration on Biodiversity Conservation in Karst Mountain Area of Southwest of China, School of Life Science, Guizhou Normal University, Guiyang, China.
Pharmacy College, Guizhou University of Traditional Chinese Medicine, Guiyang, China.
Front Plant Sci. 2022 May 16;13:863482. doi: 10.3389/fpls.2022.863482. eCollection 2022.
Flower color, largely determined by anthocyanin, is one of the most important ornamental values of . However, scant information of anthocyanin biosynthesis has been reported in . We found that anthocyanidin 3--glycosides were the predominant anthocyanins detected in flowers accounting for 93.68-96.31% of the total anthocyanins during its development, which indicated the key role of flavonoid 3--glycosyltransferase (3GT) on flower color formation. Subsequently, based on correlation analysis between anthocyanins accumulation and expressions during flower development, and were preliminarily identified as the pivotal genes involved in the formation of color of flower. Tissue-specific expressions of and were examined, and their function as 3GT was confirmed through introducing into mutant and plants. Furthermore, biochemical characterizations showed that both and could catalyze the addition of UDP-sugar to the 3-OH of anthocyanidin, and preferred UDP-Gal as their sugar donor and cyanidin as the most efficient substrate. This study not only provides insights into the biosynthesis of anthocyanin in , but also makes contribution to understand the mechanisms of its flower color formation.
花色在很大程度上由花青素决定，是[植物名称]最重要的观赏价值之一。然而，关于[植物名称]花青素生物合成的信息报道较少。我们发现花青素3 - [糖苷化修饰部分] - 糖苷是在[植物名称]花朵中检测到的主要花青素，在其发育过程中占总花青素的93.68 - 96.31%，这表明类黄酮3 - [糖苷化修饰部分] - 糖基转移酶（3GT）在[植物名称]花色形成中起关键作用。随后，基于花朵发育过程中花青素积累与[基因名称]表达之间的相关性分析，[基因名称1]和[基因名称2]被初步鉴定为参与[植物名称]花色形成的关键基因。检测了[基因名称1]和[基因名称2]的组织特异性表达，并通过导入[植物名称]突变体和[植物名称]植株证实了它们作为3GT的功能。此外，生化特性表明，[基因名称1]和[基因名称2]都可以催化将UDP - 糖添加到花青素的3 - OH上，并且优先选择UDP - Gal作为糖供体，矢车菊素作为最有效的底物。本研究不仅为[植物名称]花青素的生物合成提供了见解，也为理解其花色形成机制做出了贡献。
