Zhou Lin, Chen Xiaojuan, Su Wenkun, Ou Zhi, Qu Yan
Southwest Research Center for Engineering Technology of Landscape Architecture (State Forestry and Grassland Administration), Yunnan Engineering Research Center for Functional Flower Resources and Industrialization, College of Landscape Architecture and Horticulture Science, Southwest Forestry University, Kunming 650224, China.
Plants (Basel). 2025 Mar 17;14(6):944. doi: 10.3390/plants14060944.
Flower color is one of the most ornamental values of , but very limited studies have been reported on its flower color formation. The UDP-glycosyltransferase (UGT) gene family plays a crucial role in plant flower color formation. In this study, the full-length transcriptome data of was used to identify MwUGTs, focusing on protein physicochemical properties' subcellular localization, and phylogenetic relationships. In addition, sequence analysis, expression pattern analysis, subcellular localization, and functional validation of were also performed. A total of 26 MwUGTs were identified in full-length transcriptome and clustered into eight subgroups. Phylogenetic analysis and KEGG database annotation showed that is associated with anthocyanin synthesis and accumulation. Subsequently, based on the expression of during flower development and in different tissues, it was preliminarily determined that MwUGT2 plays a role in the flower bud stage. Subcellular localization assays suggested that is present in the nucleus and cytoplasm. Overexpression in showed that significantly increased the content of Cyanidin-3-O-glucoside and resulted in dark pink flowers in transgenic plants. In summary, our findings suggest that plays a crucial role in the biosynthesis of anthocyanin and will also contribute to understanding the mechanisms of flower color formation in .
花色是[植物名称]最具观赏价值的特征之一，但关于其花色形成的研究报道非常有限。UDP-糖基转移酶（UGT）基因家族在植物花色形成中起着关键作用。在本研究中，利用[植物名称]的全长转录组数据来鉴定MwUGT，重点关注蛋白质的理化性质、亚细胞定位和系统发育关系。此外，还对[相关基因]进行了序列分析、表达模式分析、亚细胞定位和功能验证。在全长转录组中总共鉴定出26个MwUGT，并将其聚类为八个亚组。系统发育分析和KEGG数据库注释表明，[相关基因]与花青素的合成和积累有关。随后，基于[相关基因]在花发育过程中和不同组织中的表达，初步确定MwUGT2在花芽阶段发挥作用。亚细胞定位分析表明，[相关基因]存在于细胞核和细胞质中。在[植物名称]中过表达表明，[相关基因]显著增加了矢车菊素-3-O-葡萄糖苷的含量，并导致转基因植物的花朵呈现深粉色。总之，我们的研究结果表明，[相关基因]在花青素的生物合成中起着关键作用，也将有助于理解[植物名称]花色形成的机制。
