Alimi Olawale Alimi, Abubakar Adamu Abdul, Yakubu Abubakar Sadiq, Shehu Sani Abdullahi, Abdulkadir Salman Zubairu, Oviawe Ekaete Ime
Departamento de Cirurgia e Radiologia Veterinária, University of Ilorin, Ilorin, Nigéria.
Departamento de Cirurgia e Radiologia Veterinária, Usmanu Danfodiyo University, Sokoto, Nigéria.
Rev Bras Ortop (Sao Paulo). 2021 Aug 13;57(2):218-222. doi: 10.1055/s-0041-1729592. eCollection 2022 Apr.
Several animal models have been used in fracture healing and bone graft studies, but hematological responses are seldom reported. Therefore, the present study reported the hematological changes observed in rabbits that underwent xenografting of caprine demineralized bone matrix (CDBM). Twenty-four (24) male rabbits (2.5 ± 0.5kg) were acquired for the purpose of this study and were randomly assigned to three groups: autologous bone graft (ABG), unfilled (NC), and caprine demineralized bone matrix (CDBM). Blood samples were collected through cardiac puncture under xylazine-ketamine anesthesia on day 0 (baseline), and on days 28 and 56 postsurgery and were analyzed manually within 2 hours of collection. Statistical analysis was performed using a two-way analysis of variance (ANOVA) with repeated measures, and a -value < 0.05 was considered significant. There was an overall significant difference in the values of total white blood cell count ( = 0.0043), neutrophil count ( < 0.0001), monocyte count ( = 0.0184), red blood cell count ( = 0.003), hemoglobin concentration ( < 0.0001) and packed cell volume ( < 0.0001) across the days and the treatment groups. There was, however, no overall significant difference in lymphocyte count ( = 0.4923), basophil count ( = 0.4183), and eosinophil count (0.4806) within days. Response to CDBM grafting in rabbits could, therefore, be said to be characterized by marked leukocytosis with neutrophilia, lymphocytosis, and monocytosis by day 28 of postgrafting. This could form the basis with which hematology can be used to monitor body response of bone graft animal models.
几种动物模型已用于骨折愈合和骨移植研究，但血液学反应鲜有报道。因此，本研究报告了接受羊脱矿骨基质（CDBM）异种移植的兔子的血液学变化。
本研究共获取24只雄性兔子（体重2.5±0.5千克），并将它们随机分为三组：自体骨移植组（ABG）、空白组（NC）和羊脱矿骨基质组（CDBM）。在第0天（基线）、术后第28天和第56天，于赛拉嗪-氯胺酮麻醉下通过心脏穿刺采集血样，并在采集后2小时内进行手工分析。采用重复测量的双向方差分析进行统计分析，P值<0.05被认为具有统计学意义。
总体而言，不同天数和治疗组之间的白细胞总数（P = 0.0043）、中性粒细胞计数（P < 0.0001）、单核细胞计数（P = 0.0184）、红细胞计数（P = 0.003）、血红蛋白浓度（P < 0.0001）和血细胞比容（P < 0.0001）值存在显著差异。然而，不同天数内淋巴细胞计数（P = 0.4923）、嗜碱性粒细胞计数（P = 0.4183）和嗜酸性粒细胞计数（P = 0.4806）无总体显著差异。
因此，可以说兔子对CDBM移植的反应特征是移植后第28天出现明显的白细胞增多，并伴有中性粒细胞增多、淋巴细胞增多和单核细胞增多。这可以作为血液学用于监测骨移植动物模型机体反应的基础。
