Hayat Shoma, Asad Asaduzzaman, Hasan Imran, Jahan Israt, Papri Nowshin, Howlader Zakir Hossain, Islam Zhahirul
Laboratory of Gut-Brain Signaling, Laboratory Sciences and Services Division (LSSD), Dhaka, Bangladesh.
Department of Biochemistry and Molecular Biology, University of Dhaka, Dhaka, Bangladesh.
Acta Neurol Scand. 2022 Aug;146(2):177-185. doi: 10.1111/ane.13649. Epub 2022 Jun 2.
Nucleotide oligomerization domain (NOD) proteins are cytoplasmic receptors that play important roles in host innate immune responses to pathogens by recognizing self or non-self-molecules and have been implicated in many autoimmune diseases including Guillain-Barré syndrome (GBS). The current study investigated whether NOD polymorphisms (NOD1-Glu266Lys, rs2075820, and NOD2- [Arg702Trp, rs2066844 and Gly908Arg, rs2066845]) contribute to ligand sensing and thus affect the susceptibility and/or severity of GBS.
We determined single nucleotide polymorphisms (SNPs) of NOD gene (NOD1-Glu266Lys and NOD2-[Arg702Trp; Gly908Ar]) in 303 patients with GBS and 303 healthy controls from Bangladesh by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and Sanger sequencing. Genotypes and allele frequencies were compared by performing chi-squared or Fisher's exact test with Yates' continuity correction. Serology for Campylobacter jejuni and anti-GM1 antibodies were determined by enzyme-linked immunosorbent assay (ELISA) techniques.
NOD variants (NOD1-Glu266Lys and NOD2- [Arg702Trp; Gly908Arg]) were not associated with susceptibility and severity of GBS when compared with healthy controls and mild or severe form of disease. Moreover, NOD2 polymorphisms showed wild-type NOD2 C2104 and NOD2 G2722, respectively, with homozygous Arg/Arg genotype of NOD2 (Arg702Trp) polymorphism and homozygous Gly/Gly genotype of NOD2 (Gly908Arg) for all study subjects in Bangladesh. Homogenous distribution of NOD1 genotypes was observed in patients with axonal and demyelinating form of GBS.
NOD variants confer no risk to the susceptibility and severity of GBS. Moreover, NOD2 polymorphism is rare or absent in patients with GBS as well as in the healthy individuals of Bangladesh.
核苷酸寡聚化结构域（NOD）蛋白是细胞质受体，通过识别自身或非自身分子在宿主对病原体的固有免疫反应中发挥重要作用，并且与包括吉兰 - 巴雷综合征（GBS）在内的许多自身免疫性疾病有关。本研究调查了NOD基因多态性（NOD1 - Glu266Lys，rs2075820，以及NOD2 - [Arg702Trp，rs2066844和Gly908Arg，rs2066845]）是否有助于配体感知，从而影响GBS的易感性和/或严重程度。
我们通过聚合酶链反应 - 限制性片段长度多态性（PCR - RFLP）和桑格测序法，确定了来自孟加拉国的303例GBS患者和303名健康对照者中NOD基因的单核苷酸多态性（SNPs）（NOD1 - Glu266Lys和NOD2 - [Arg702Trp；Gly908Ar]）。通过进行卡方检验或采用耶茨连续性校正的费舍尔精确检验来比较基因型和等位基因频率。采用酶联免疫吸附测定（ELISA）技术检测空肠弯曲菌血清学和抗GM1抗体。
与健康对照者以及疾病的轻度或重度形式相比，NOD变体（NOD1 - Glu266Lys和NOD2 - [Arg702Trp；Gly908Arg]）与GBS的易感性和严重程度无关。此外，在孟加拉国的所有研究对象中，NOD2多态性分别显示野生型NOD2 C2104和NOD2 G2722，NOD2（Arg702Trp）多态性为纯合Arg/Arg基因型，NOD2（Gly908Arg）为纯合Gly/Gly基因型。在轴索性和脱髓鞘性GBS患者中观察到NOD1基因型的均匀分布。
NOD变体对GBS的易感性和严重程度没有风险。此外，在孟加拉国的GBS患者以及健康个体中，NOD2多态性很少见或不存在。
