Laboratory of Plant Improvement and Agro-Resources Valorization, National School of Engineers of Sfax, road of Soukra Km 4, B.P 1173, 3038, Sfax, Tunisia.
Laboratory of Environmental Bioprocesses, Sfax Biotechnology Center, Road of Sidi Mansour km6, BP 1177, 3018, Sfax, Tunisia.
Environ Sci Pollut Res Int. 2022 Oct;29(50):75365-75379. doi: 10.1007/s11356-022-21046-8. Epub 2022 Jun 2.
The beneficial effect of compost and compost tea on plant growth and protection is mainly associated with the microbial diversity and the presence of bacteria with plant growth-promoting effect. PGPR are considered as eco-friendly bio-fertilizers that may reduce the use of chemical pesticides and fertilizers. Three composts (AT, A10, and A30) were previously prepared from industrial wastes (olive mill wastewater, olive pomace, coffee ground, and phosphogypsum). In the present study, we isolated three bacterial strains from the compost teas. The phylogenetic identification of these bacterial strains (B.AT, B.A10, and B.A30) showed that they correspond to Serratia liquefaciens (B.AT and B.A10) and Achromobacter spanius (B.A30) species. A further characterization of the PGPR traits of these bacteria showed that they produce siderophore, exopolysaccharides, and IAA. Their effect on potato plant growth, yields, and tuber quality was performed under field culture conditions. Results showed that these strains can be characterized as PGPR, the best effect on potato plant growth was observed with Serratia liquefaciens (B.AT), the best yield and tuber quality was observed with Serratia liquefaciens (B.A10) while bacterial treatment with Achromobacter spanius (B.A30) is a Cd-tolerant PGPR.
堆肥和堆肥茶对植物生长和保护的有益影响主要与微生物多样性和具有促进植物生长效应的细菌的存在有关。PGPR 被认为是环保型生物肥料，可减少化学农药和化肥的使用。先前已使用工业废物（橄榄磨废水、橄榄渣、咖啡渣和磷石膏）制备了三种堆肥（AT、A10 和 A30）。在本研究中，我们从堆肥茶中分离出了三株细菌菌株。这些细菌菌株（B.AT、B.A10 和 B.A30）的系统发育鉴定表明，它们对应于液化沙雷氏菌（B.AT 和 B.A10）和黄杆菌属（B.A30）物种。对这些细菌的 PGPR 特性的进一步表征表明，它们产生铁载体、胞外多糖和 IAA。在田间培养条件下，研究了它们对马铃薯植物生长、产量和块茎质量的影响。结果表明，这些菌株可被表征为 PGPR，其中液化沙雷氏菌（B.AT）对马铃薯植物生长的影响最好，液化沙雷氏菌（B.A10）的产量和块茎质量最好，而黄杆菌属（B.A30）的细菌处理是一种 Cd 耐受 PGPR。
