脐带血清 miRNA 与儿童肥胖相关性的研究：来自出生队列研究的初步研究。
Investigation of umbilical cord serum miRNAs associated with childhood obesity: A pilot study from a birth cohort study.
机构信息
Department of Sustainable Health Science, Center for Preventive Medical Sciences, Chiba University, Chiba, Japan.
Department of Molecular and Cellular Medicine, Institute of Medical Science, Tokyo Medical University, Tokyo, Japan.
出版信息
J Diabetes Investig. 2022 Oct;13(10):1740-1744. doi: 10.1111/jdi.13863. Epub 2022 Jun 17.
We investigated umbilical cord serum microRNA (miRNA) profiles to identify biomarkers of a risk for obesity later in life. Participating children were divided into high- and low-risk groups of obesity based on the timing of adiposity rebound and the body mass index (BMI) at 5 years and randomly selected from each group for this study. 3D-Gene® Human miRNA Oligo Chip was performed using cord serum in five children of both groups. The most relevant miRNAs were confirmed in 33 children of the groups using the TaqMan® microRNA assay. We detected five cord serum miRNAs differentially expressed in children at high risk of obesity compared with the levels in children at low risk, namely, miR-516-3p and miR-130a-3p with increased levels and miR-1260b, miR-4709-3p, and miR194-3p with decreased levels. This study provides the first identification of altered umbilical cord serum miRNAs in childhood obesity.
我们研究了脐带血清 microRNA（miRNA）谱，以鉴定与日后肥胖相关的生物标志物。根据肥胖反弹的时间和 5 岁时的体重指数（BMI），将参与的儿童分为肥胖高风险和低风险组，并从每组中随机选择进行本研究。使用两组中各 5 名儿童的脐带血清进行了 3D-Gene® Human miRNA Oligo Chip 实验。使用 TaqMan® microRNA 分析在两组的 33 名儿童中确认了最相关的 miRNAs。与低风险儿童相比，我们在肥胖高风险儿童的脐带血清中检测到了五个差异表达的 miRNA，即 miR-516-3p 和 miR-130a-3p 水平升高，miR-1260b、miR-4709-3p 和 miR194-3p 水平降低。本研究首次鉴定了儿童肥胖症中改变的脐带血清 miRNAs。
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