Department of Sustainable Health Science, Center for Preventive Medical Sciences, Chiba University, Chiba, Japan.
Department of Molecular and Cellular Medicine, Institute of Medical Science, Tokyo Medical University, Tokyo, Japan.
J Diabetes Investig. 2022 Oct;13(10):1740-1744. doi: 10.1111/jdi.13863. Epub 2022 Jun 17.
We investigated umbilical cord serum microRNA (miRNA) profiles to identify biomarkers of a risk for obesity later in life. Participating children were divided into high- and low-risk groups of obesity based on the timing of adiposity rebound and the body mass index (BMI) at 5 years and randomly selected from each group for this study. 3D-Gene® Human miRNA Oligo Chip was performed using cord serum in five children of both groups. The most relevant miRNAs were confirmed in 33 children of the groups using the TaqMan® microRNA assay. We detected five cord serum miRNAs differentially expressed in children at high risk of obesity compared with the levels in children at low risk, namely, miR-516-3p and miR-130a-3p with increased levels and miR-1260b, miR-4709-3p, and miR194-3p with decreased levels. This study provides the first identification of altered umbilical cord serum miRNAs in childhood obesity.
我们研究了脐带血清 microRNA（miRNA）谱，以鉴定与日后肥胖相关的生物标志物。根据肥胖反弹的时间和 5 岁时的体重指数（BMI），将参与的儿童分为肥胖高风险和低风险组，并从每组中随机选择进行本研究。使用两组中各 5 名儿童的脐带血清进行了 3D-Gene® Human miRNA Oligo Chip 实验。使用 TaqMan® microRNA 分析在两组的 33 名儿童中确认了最相关的 miRNAs。与低风险儿童相比，我们在肥胖高风险儿童的脐带血清中检测到了五个差异表达的 miRNA，即 miR-516-3p 和 miR-130a-3p 水平升高，miR-1260b、miR-4709-3p 和 miR194-3p 水平降低。本研究首次鉴定了儿童肥胖症中改变的脐带血清 miRNAs。
