带状狗鲨血清中的鸡型溶菌酶是一种主要的溶菌酶。
Chicken-type lysozyme is a major bacteriolytic enzyme in the blood of the banded houndshark Triakis scyllium.
机构信息
Laboratory of Genome Science, Graduate School of Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo, 108-8477, Japan.
Laboratory of Genome Science, Graduate School of Tokyo University of Marine Science and Technology, Konan 4-5-7, Minato-ku, Tokyo, 108-8477, Japan.
出版信息
Dev Comp Immunol. 2022 Sep;134:104448. doi: 10.1016/j.dci.2022.104448. Epub 2022 May 30.
We examined lysozyme activities in the serum and the leukocyte extracts of the banded houndshark Triakis scyllium. The serum exhibited lytic activity, but not the leukocyte extracts. The lytic substance in the serum was of approximately 14 kDa and the N-terminal amino acid sequence was YVYSK. cDNA cloning identified a C-type lysozyme (TsLysC) gene and two G-type lysozyme (TsLysG) cDNA clones of different lengths. The TsLysC gene encodes 149 amino acids residues, and the sequence derived from the N-terminal amino acid sequencing was displayed at position 17-21. TsLysG, on the other hand, contains two ORFs that are homologous to the N- and C-terminal regions of G-type lysozyme of other fish species. TsLysC mRNA levels were high in the liver. TsLysG mRNA level was significantly lower than TsLysC mRNA in the liver.
我们研究了条纹斑竹鲨（Triakis scyllium）血清和白细胞提取物中的溶菌酶活性。血清具有溶菌活性，但白细胞提取物则没有。血清中的溶菌物质约为 14 kDa，其 N 端氨基酸序列为 YVYSK。通过 cDNA 克隆鉴定出一个 C 型溶菌酶（TsLysC）基因和两个长度不同的 G 型溶菌酶（TsLysG）cDNA 克隆。TsLysC 基因编码 149 个氨基酸残基，从 N 端氨基酸测序得出的序列显示在 17-21 位。另一方面，TsLysG 包含两个 ORF，与其他鱼类 G 型溶菌酶的 N 和 C 末端区域同源。TsLysC mRNA 在肝脏中表达水平较高，而 TsLysG mRNA 在肝脏中的表达水平显著低于 TsLysC mRNA。
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