Research Center of Chinese Herbal Resource Science and Engineering, Guangzhou University of Chinese Medicine; Key Laboratory of Chinese Medicinal Resource from Lingnan (Guangzhou University of Chinese Medicine), Ministry of Education; Joint Laboratory of National Engineering Research Center for the Pharmaceutics of Traditional Chinese Medicines, Guangzhou 510006, Guangdong, China.
College of Agriculture and Biology, Zhongkai University of Agriculture and Engineering, Guangzhou 510006, Guangdong, China.
J Agric Food Chem. 2022 Jun 15;70(23):7188-7201. doi: 10.1021/acs.jafc.2c01660. Epub 2022 Jun 2.
The production of patchoulol in the patchouli () plant determines its application value, as it is the principal active sesquiterpene of essential oil extracted from this plant. Here, the promoter of patchoulol synthase gene () was isolated and found to be methyl jasmonate (MeJA)-induced. A nucleus-localized AP2/ERF transcription factor PatDREB was identified as a transcription activator binding to , regulating patchoulol biosynthesis through modulating the gene expression. PatDREB also interacts with jasmonate ZIM-domain 4 (JAZ4). Furthermore, PatDREB could physically interact with the MYB-related transcription factor PatSWC4 and synergistically facilitate patchoulol biosynthesis. However, the transcriptional activation activity of the PatDREB-PatSWC4 complex could be inhibited by PatJAZ4, and JA could reverse this interference. Overall, we demonstrated the positive roles of PatDREB and the PatDREB-PatSWC4 complex in regulating patchoulol production, which advance our understanding of the regulatory network of patchoulol biosynthesis.
姜酮的合成在广藿香植物中决定了其应用价值，因为它是从这种植物中提取的精油的主要活性倍半萜烯。在这里，我们分离到了姜酮合酶基因（）的启动子，发现它受茉莉酸甲酯（MeJA）诱导。鉴定到一个定位于细胞核的 AP2/ERF 转录因子 PatDREB 作为一个转录激活因子结合到，通过调节基因表达来调控姜酮的生物合成。PatDREB 还与茉莉酸 ZIM 结构域蛋白 4（JAZ4）相互作用。此外，PatDREB 可以与 MYB 相关的转录因子 PatSWC4 相互作用，并协同促进姜酮的生物合成。然而，PatDREB-PatSWC4 复合物的转录激活活性可以被 PatJAZ4 抑制，而 JA 可以逆转这种干扰。总的来说，我们证明了 PatDREB 和 PatDREB-PatSWC4 复合物在调节姜酮生物合成中的积极作用，这推进了我们对姜酮生物合成调控网络的理解。
