Shi Jianling, Cui Yingjing, Zhang Jimeng, Sun Liqiong, Tang Xiaoqing
Institute of Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, China.
Curr Issues Mol Biol. 2023 Mar 24;45(4):2738-2756. doi: 10.3390/cimb45040179.
Methyl jasmonate has an important effect on the synthesis of plant secondary metabolites. Briq. has a wide range of pharmacological effects and the secondary metabolites are dominated by monoterpenes (pulegone, menthone).
It is essential to determine the changes in secondary metabolites in under methyl jasmonate treatment and to probe the molecular mechanism. This can improve the accumulation of secondary metabolites in the medicinal plant and enrich the information gene expression at different MeJA levels, which can help to elucidate the molecular mechanism of monoterpenoid synthesis in .
In this study, we determined the changes in the content of monoterpenoids in under methyl jasmonate treatment. Meanwhile, we established a transcriptome database of under methyl jasmonate level using high-throughput sequencing.
A certain concentration of MeJA promoted the accumulation of monoterpenoids in . The transcriptome database of leaves under 0, 50, 100 and 250 μM MeJA treatment was established. We generated 88,373 unigenes with an N50 length of 2678 bp, of which 50,843 (57.53%) can be annotated in at least one database. Compared with the CK (0 μM) group, 12,557 (50 μM), 15,409 (100 μM) and 13,286 (250 μM) differentially expressed genes were identified. GO and KEGG enrichment analysis revealed that JA signal transduction and monoterpenoid synthesis were the two most significant enrichment pathways. The expression levels of related DEGs involved in JA signaling and monoterpenoid synthesis were significantly up-regulated by MeJA. In addition, our phenotypic and differentially expressed gene association analysis revealed that monoterpenoid biosynthesis in was more associated with genes involved in plant trichome branching, phytohormone signaling and transcriptional regulation.
This study confirmed that methyl jasmonate significantly promoted monoterpenoid biosynthesis in . A large number of genes responding to methyl jasmonate were associated with JA signaling and monoterpenoid biosynthesis.
茉莉酸甲酯对植物次生代谢产物的合成具有重要作用。Briq. 具有广泛的药理作用，其次生代谢产物以单萜类化合物（胡薄荷酮、薄荷酮）为主。
确定茉莉酸甲酯处理下Briq. 中次生代谢产物的变化，并探究其分子机制。这可以提高药用植物Briq. 中次生代谢产物的积累，并丰富不同茉莉酸甲酯水平下的信息基因表达，有助于阐明Briq. 中单萜类化合物合成的分子机制。
在本研究中，我们测定了茉莉酸甲酯处理下Briq. 中单萜类化合物含量的变化。同时，我们利用高通量测序建立了不同茉莉酸甲酯水平下Briq. 的转录组数据库。
一定浓度的茉莉酸甲酯促进了Briq. 中单萜类化合物的积累。建立了0、50、100和250 μM茉莉酸甲酯处理下Briq. 叶片的转录组数据库。我们生成了88373个单基因，N50长度为2678 bp，其中50843个（57.53%）可以在至少一个数据库中注释。与CK（0 μM）组相比，分别鉴定出12557个（50 μM）、15409个（100 μM）和13286个（250 μM）差异表达基因。GO和KEGG富集分析表明，茉莉酸信号转导和单萜类化合物合成是两个最显著的富集途径。茉莉酸甲酯显著上调了参与茉莉酸信号转导和单萜类化合物合成的相关差异表达基因的表达水平。此外，我们的表型和差异表达基因关联分析表明，Briq. 中单萜类化合物的生物合成与参与植物毛状体分支、植物激素信号转导和转录调控的基因更相关。
本研究证实茉莉酸甲酯显著促进了Briq. 中单萜类化合物的生物合成。大量响应茉莉酸甲酯的基因与茉莉酸信号转导和单萜类化合物生物合成相关。
