Harkema J R, Plopper C G, Hyde D M, St George J A, Dungworth D L
Am J Pathol. 1987 Apr;127(1):90-6.
Despite the absorption of inhaled oxidant gases by the nasal cavity, little effort has been made to characterize the effects of these oxidants on the nasal mucosa. This study defines the effects of ambient concentrations of ozone on the character and amount of mucosubstances in epithelium of nasal mucosa. Bonnet monkeys were exposed to 0.00 or 0.15 ppm O3 (8 hr/day) for 6 or 90 days, anesthetized, and exsanguinated. Nasal cavities were fixed with Karnovsky's fixative, decalcified, and processed for light microscopy, and sections were stained with alcian blue (pH 2.5)/periodic acid-Schiff or high iron diamine. Volume densities of secretory material in nasal epithelium were determined with the use of a Quantimet 900 image analyzer. After 6 days' exposure there were significant increases in both acidic and neutral glycoconjugates stored in transitional and respiratory epithelium. After 90 days there was significantly less mucosubstance than at 6 days. Only in the transitional epithelium did the total and sulfated mucosubstance remain greater than that of controls. Nasopharyngeal epithelium was minimally affected after 6 days of O3 and unchanged after 90 days. It is concluded that exposures to ambient levels of O3 induce significant changes in the stored secretory product of nasal epithelium.
尽管鼻腔会吸收吸入的氧化性气体，但人们很少致力于描述这些氧化剂对鼻黏膜的影响。本研究确定了环境浓度的臭氧对鼻黏膜上皮中黏液物质的特征和数量的影响。将帽猴暴露于0.00或0.15 ppm的臭氧中（每天8小时），持续6天或90天，然后对其进行麻醉并放血。将鼻腔用卡诺夫斯基固定液固定，脱钙，然后进行光学显微镜检查处理，切片用阿尔辛蓝（pH 2.5）/过碘酸-希夫试剂或高铁二胺染色。使用Quantimet 900图像分析仪测定鼻上皮中分泌物质的体积密度。暴露6天后，过渡性和呼吸性上皮中储存的酸性和中性糖缀合物均显著增加。90天后，黏液物质比6天时显著减少。只有在过渡性上皮中，总黏液物质和硫酸化黏液物质仍高于对照组。臭氧暴露6天后，鼻咽上皮受到的影响最小，90天后无变化。研究得出结论，暴露于环境水平的臭氧会导致鼻上皮中储存的分泌产物发生显著变化。
