Lahimgarzadeh Roya, Vaseghi Salar, Nasehi Mohammad, Rouhollah Fatemeh
Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.
Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran.
Iran J Basic Med Sci. 2022 Feb;25(2):164-172. doi: 10.22038/IJBMS.2022.61175.13536.
Sleep deprivation (SD) has a negative impact on cognitive functions including learning and memory. Many studies have shown that rapid-eye-movement (REM) SD also disrupts memory performance. In this study, we aimed to investigate the effect of multi-epitope Gag-Pol-Env-Tat derived from Human immunodeficiency virus 1 (HIV-1) on REM SD-induced spatial memory impairment with respect to the levels of interleukin-4 (IL-4), interleukin-17 (IL-17), interferon-gamma (IFN-γ), immunoglobulin G1 (IgG1), immunoglobulin G2a (IgG2a), and lymphocyte proliferation in NMRI mice. We used multi-epitope Gag-Pol-Env-Tat derived from HIV-1 because Gag-Pol-Env-Tat immunogen sequence is one of the most sensitive immunogen sequences of HIV-1 that can significantly augment cellular and humoral immune systems, leading to the improvement of cognitive functions.
Morris water maze apparatus was used to assess spatial memory, and multi-platform apparatus was used to induce RSD for 24 hr. Multi-epitope derived from HIV-1 was subcutaneously injected at the dose of 20 µgr/ml, once and fourteen days before RSD.
RSD impaired spatial memory and injection of multi-epitope derived from HIV-1 reversed this effect. RSD decreased IL-4, IgG1, and IgG2a levels, while multi-epitope derived from HIV-1 reversed these effects. Multi-epitope derived from HIV-1 also increased lymphocyte proliferation and decreased IL-17 levels in both control and RSD mice.
Multi-epitope derived from HIV-1 may improve memory performance via induction of anti-inflammatory immune response.
睡眠剥夺（SD）对包括学习和记忆在内的认知功能有负面影响。许多研究表明，快速眼动（REM）睡眠剥夺也会破坏记忆表现。在本研究中，我们旨在研究源自人类免疫缺陷病毒1型（HIV-1）的多表位Gag-Pol-Env-Tat对REM睡眠剥夺诱导的空间记忆损害的影响，涉及NMRI小鼠体内白细胞介素-4（IL-4）、白细胞介素-17（IL-17）、干扰素-γ（IFN-γ）、免疫球蛋白G1（IgG1）、免疫球蛋白G2a（IgG2a）水平以及淋巴细胞增殖情况。我们使用源自HIV-1的多表位Gag-Pol-Env-Tat，是因为Gag-Pol-Env-Tat免疫原序列是HIV-1最敏感的免疫原序列之一，可显著增强细胞免疫和体液免疫系统，从而改善认知功能。
使用Morris水迷宫装置评估空间记忆，使用多平台装置诱导24小时的快速眼动睡眠剥夺（RSD）。以20µgr/ml的剂量皮下注射源自HIV-1的多表位，分别在RSD前1天和14天各注射一次。
RSD损害了空间记忆，而注射源自HIV-1的多表位可逆转这种效应。RSD降低了IL-4、IgG1和IgG2a水平，而源自HIV-1的多表位可逆转这些效应。源自HIV-1的多表位还增加了对照组和RSD小鼠的淋巴细胞增殖，并降低了IL-17水平。
源自HIV-1的多表位可能通过诱导抗炎免疫反应来改善记忆表现。
