Shabar Hamza, DiAngelo Justin R
Division of Science, Penn State Berks, Reading, PA, USA.
MicroPubl Biol. 2022 May 30;2022. doi: 10.17912/micropub.biology.000580. eCollection 2022.
Excess triglycerides from the diet are stored in structures called lipid droplets in adipose tissue. Genome-wide RNAi screens have identified mRNA splicing factors as important for lipid droplet formation; however, the full complement of splicing factors that regulate lipid storage is not known. Here, we characterize the role of , the gene encoding for a splicing protein involved in recognizing the 5' splice site in introns, in regulating lipid and carbohydrate storage in the fat body. Decreasing specifically in the fly fat body resulted in less triglyceride, glycogen, and glucose in each fat body cell. Consistent with these decreased nutrient storage phenotypes, flies ate less, providing a potential cause for less lipid and carbohydrate storage in these flies. These data further support the role of mRNA processing in regulating metabolic homeostasis in .
饮食中过量的甘油三酯会储存在脂肪组织中称为脂滴的结构中。全基因组RNA干扰筛选已确定mRNA剪接因子对脂滴形成很重要；然而，调节脂质储存的剪接因子的完整组成尚不清楚。在这里，我们描述了 （一种参与识别内含子中5'剪接位点的剪接蛋白的编码基因）在调节果蝇脂肪体中脂质和碳水化合物储存方面的作用。在果蝇脂肪体中特异性降低 会导致每个脂肪体细胞中的甘油三酯、糖原和葡萄糖减少。与这些营养储存减少的表型一致， 果蝇吃得更少，这为这些果蝇中脂质和碳水化合物储存减少提供了一个潜在原因。这些数据进一步支持了mRNA加工在调节果蝇代谢稳态中的作用。
