Sabzehali Fattaneh, Goudarzi Hossein, Goudarzi Mehdi, Salimi Chirani Alireza, Yoosefi Izad Mohammad Hossein
Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Iran J Basic Med Sci. 2022 Mar;25(3):276-285. doi: 10.22038/IJBMS.2022.61448.13595.
, as an opportunistic pathogen, is known to cause nosocomial infections among patients suffering from burn injuries and also cystic fibrosis patients. The objective of our research was to develop a novel vaccine against .
A recombinant subunit vaccine based on the outer membrane proteins, including the OprF-OprI region and its major protein in the type III secretion system, PopB (called FIB protein) was formulated. To induce a robust immune response, our preferred regions were conjugated to a carrier protein, GMCSF (Granulocyte-macrophage colony-stimulating factor). FIB protein's immunogenicity with and without adjuvant was evaluated in vaccinated rats and also burned rat models, which were subcutaneously challenged by the PAO1 strain of .
Antibody levels were increased in sera of rats in this study. Assessment of the resident memory CD4+ T cells in splenocytes from vaccinated rats demonstrated that the FIB conjugated with GMCSF could cause higher responses in comparison with other groups. Moreover, immunization with the FIB plus adjuvant protein could improve interferon-gamma (IFN-γ) production, interleukin 17A (IL-17A), and IL-4, contributing to elicit humoral and cellular immune responses and decreased post-infection bacterial loads after PA challenge, pathology, and inflammatory cell infiltration.
These observations demonstrated that FIB conjugated with GMCSF can be a putative vaccine candidate against in burnt rat models.
作为一种机会致病菌，已知会在烧伤患者以及囊性纤维化患者中引起医院感染。我们研究的目的是开发一种针对[病原体名称未给出]的新型疫苗。
制备了一种基于外膜蛋白的重组亚单位疫苗，包括OprF - OprI区域及其III型分泌系统中的主要蛋白PopB（称为FIB蛋白）。为诱导强烈的免疫反应，我们将优选区域与载体蛋白GMCSF（粒细胞 - 巨噬细胞集落刺激因子）偶联。在接种疫苗的大鼠以及烧伤大鼠模型中评估了有无佐剂情况下FIB蛋白的免疫原性，这些大鼠通过[病原体名称未给出]的PAO1菌株进行皮下攻击。
本研究中大鼠血清中的抗体水平升高。对接种疫苗大鼠脾细胞中驻留记忆CD4 + T细胞的评估表明，与GMCSF偶联的FIB与其他组相比可引起更高的反应。此外，用FIB加佐剂蛋白免疫可提高干扰素 - γ（IFN - γ）、白细胞介素17A（IL - 17A）和IL - 4的产生，有助于引发体液和细胞免疫反应，并降低PA攻击后感染后的细菌载量、病理学改变和炎性细胞浸润。
这些观察结果表明，与GMCSF偶联的FIB在烧伤大鼠模型中可能是一种针对[病原体名称未给出]的候选疫苗。
