Autoimmunity, Cancer, And Immunogenetics Research Laboratory, University Hospital Habib Bourguiba of Sfax, Tunisia.
Pediatrics Department, University Hospital Hedi Chaker of Sfax, Tunisia.
J Diabetes Res. 2022 May 23;2022:2539871. doi: 10.1155/2022/2539871. eCollection 2022.
Type 1 diabetes (T1D) occurs as a result of insulin deficiency due to destructive lesions of pancreatic cells. In addition to classical autoantibodies (Abs) to islet cell antigens, antizinc transporter 8 Abs (ZnT8-Ab) have been recently described in T1D.
As no data on ZnT8-Ab in Tunisian patients has been reported, we aim to evaluate the relationships between ZnT8-Ab, ZnT8 coding gene () promoter polymorphism, and T1D risk in newly diagnosed children.
ZnT8-Ab were measured in the serum of T1D newly affected children ( = 156) who were admitted to the pediatric department of the Hedi Chaker University Hospital of Sfax. Rs13266634 was genotyped in T1D children and 79 of their first-degree parents. The SPSS software was used to analyze the serological data. Allelic association analysis was conducted with family-based association tests implemented in the FBAT program v1.5.1.
ZnT8-Ab was detected in 66/156 (42.3%) of T1D newly diagnosed children. Among them, 6 (9%) presented ZnT8-Ab as the only humoral marker. The inclusion of ZnT8-Ab increased the number of Ab-positive patients to 90% and reduced the negative ones by 27%. There was no evidence of any overtransmission of any allele of the rs13266634 C/T polymorphism from parents to affected T1D children, nor of any correlation with any clinical or serological parameter. After the T1D disease onset age adjustment, a significant association was observed with the C allele suggesting that it could have a susceptibility role.
ZnT8-Ab appears as a relevant diagnostic marker for T1D in Tunisian children, especially at the onset of the disease as teenagers.
1 型糖尿病（T1D）是由于胰腺β细胞的破坏性病变导致胰岛素缺乏而发生的。除了经典的胰岛细胞抗原自身抗体（Abs）外，最近在 T1D 中还描述了抗锌转运体 8 Abs（ZnT8-Ab）。
由于尚未报道有关突尼斯患者 ZnT8-Ab 的数据，我们旨在评估 ZnT8-Ab、ZnT8 编码基因（）启动子多态性与新诊断儿童 T1D 风险之间的关系。
在入住斯法克斯赫迪·查克尔大学医院儿科病房的 156 名新确诊 T1D 患儿的血清中测量了 ZnT8-Ab。在 T1D 患儿及其 79 名一级亲属中对 Rs13266634 进行了基因分型。使用 SPSS 软件分析血清学数据。使用 FBAT 程序 v1.5.1 中实现的基于家族的关联测试进行等位基因关联分析。
在 156 名新诊断的 T1D 患儿中，有 66/156（42.3%）检测到 ZnT8-Ab。其中，6 例（9%）仅表现为 ZnT8-Ab 作为唯一的体液标志物。包含 ZnT8-Ab 将 Ab 阳性患者的数量增加到 90%，并将阴性患者减少了 27%。从父母到受影响的 T1D 儿童的 rs13266634 C/T 多态性的任何等位基因均无过度传递的证据，也与任何临床或血清学参数均无相关性。在调整 T1D 发病年龄后，与 C 等位基因观察到显著相关性，提示其可能具有易感性作用。
ZnT8-Ab 似乎是突尼斯儿童 T1D 的一个相关诊断标志物，尤其是在青少年发病时。
