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从法达马土壤中分离出的[物质名称未给出]对植物病原真菌的几丁质分解和拮抗活性 。

Chitinolytic and Antagonistic Activity of Isolated from Fadama Soil against Phytopathogenic Fungi.

作者信息

Umar Aminu Argungu, Hussaini Aminu Bandam, Yahayya Jibril, Sani Ibrahim, Aminu Habiba

机构信息

Department of Biochemistry, Kebbi State University of Science and Technology, P.M.B 1144, Aliero, Kebbi State, Nigeria.

出版信息

Trop Life Sci Res. 2021 Sep;32(3):25-38. doi: 10.21315/tlsr2021.32.3.2. Epub 2021 Sep 30.

DOI:10.21315/tlsr2021.32.3.2
PMID:35656363
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9132557/
Abstract

Chitinases which degrade chitin have attracted attention as biological antifungal agents. The purpose of this study is to isolate from Fadama soil and assess its chitinolytic and antagonist potential against phytopathogenic fungi for application as biocontrol agent. were isolated from Fadama soil. The selected isolate CT02 exhibited chitinolytic characteristics. Chitinase production was performed under different temperatures, pH and varying incubation period. The highest chitinase production by CT02 isolate was observed after five days of cultivation. The highest chitinase activity was observed at 35°C and pH 7. The crude extracellular enzyme exhibited a specific activity of 4.20 U/μg whereas partially purified extracellular enzyme exhibited a specific activity of 6.19 U/μg with purification fold of 1.47. The selected isolate CT02 and its extracellular crude chitinase showed antifungal antagonist potential by inhibiting the growth of and . This indicates that derived chitinases are potential biocontrol agents against phytopathogenic fungi.

摘要

可降解几丁质的几丁质酶作为生物抗真菌剂已引起关注。本研究的目的是从法达马土壤中分离菌株，并评估其对植物病原真菌的几丁质分解和拮抗潜力，以用作生物防治剂。从法达马土壤中分离出了菌株。所选菌株CT02表现出几丁质分解特性。在不同温度、pH值和不同培养时间下进行几丁质酶的生产。CT02菌株在培养五天后观察到最高的几丁质酶产量。在35°C和pH值为7时观察到最高的几丁质酶活性。粗细胞外酶的比活性为4.20 U/μg，而部分纯化的细胞外酶的比活性为6.19 U/μg，纯化倍数为1.47。所选菌株CT02及其细胞外粗几丁质酶通过抑制 和 的生长显示出抗真菌拮抗潜力。这表明从 中获得的几丁质酶是对抗植物病原真菌的潜在生物防治剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/612b/9132557/34236e45ae8d/tlsr-32-3-25f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/612b/9132557/9efabba6efe8/tlsr-32-3-25f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/612b/9132557/1c3bbfd20c2a/tlsr-32-3-25f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/612b/9132557/34236e45ae8d/tlsr-32-3-25f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/612b/9132557/9efabba6efe8/tlsr-32-3-25f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/612b/9132557/1c3bbfd20c2a/tlsr-32-3-25f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/612b/9132557/34236e45ae8d/tlsr-32-3-25f3.jpg

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