Gonzalez-Silva Adilene, San Juan-Mendo Magali, Delgado-Prudencio Gustavo, Hernández-García Juan Alfredo, Larios-Serrato Violeta, Aguilar César, Villa-Tanaca Lourdes, Hernández-Rodríguez César
Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Prol. Carpio y Plan de Ayala S/N, Mexico City CP 11430, Mexico.
Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca CP 62210, Mexico.
Microorganisms. 2024 Dec 19;12(12):2637. doi: 10.3390/microorganisms12122637.
is a genus of Gram-positive bacteria with high GC content. It remains attractive for studying and discovering new antibiotics, antifungals, and chemotherapeutics. genomes can contain more than 30 cryptic and expressed biosynthetic gene clusters (BGC) encoding secondary metabolites. In this study, three strains isolated from jungle rhizospheric soil exhibited supernatants that can inhibit sensitive and fluconazole-resistant spp. The genomes of the strains sp. A1, J25, J29 ori2 were sequenced, assembled de novo, and analyzed. The genome assemblies revealed that the size of the genomes was 6.9 Mb, with linear topology and 73.5% GC. A phylogenomic approach identified the strains with high similitudes between 98.5 and 98.7% with SM254 and R-53649 strains, respectively. Pangenomic analysis of eight genomes of strains deposited in the Genomes database recognized 4707 core protein orthogroups and 745 abundant accessory and exclusive protein orthogroups, suggesting an open pangenome in this species. The antiSMASH software detected candicidin and surugamide BGC-encoding polyene and octapeptide antifungal secondary metabolites in other . CORASON software was used to compare the synteny, and the abundance of genes harbored in the clusters was used. In conclusion, although the three strains belong to the same species, each possesses a distinct genome, as evidenced by the different phenotypes, including antifungal and extracellular enzymatic activities.
是一类革兰氏阳性菌，GC含量高。它在研究和发现新抗生素、抗真菌剂和化学治疗药物方面仍然具有吸引力。基因组可包含30多个编码次生代谢物的隐秘和表达的生物合成基因簇（BGC）。在本研究中，从丛林根际土壤中分离出的三株菌株表现出能够抑制敏感和氟康唑耐药的 spp. 的上清液。对菌株sp. A1、J25、J29 ori2的基因组进行了测序、从头组装和分析。基因组组装显示，基因组大小为6.9 Mb，呈线性拓扑结构，GC含量为73.5%。系统基因组学方法分别鉴定出与SM254和R-53649菌株相似度分别在98.5%至98.7%之间的高相似度菌株。对Genomes数据库中保存的八株 菌株的基因组进行泛基因组分析，识别出4707个核心蛋白质直系同源组和745个丰富的辅助和独特蛋白质直系同源组，表明该物种具有开放的泛基因组。antiSMASH软件在其他 中检测到编码多烯和八肽抗真菌次生代谢物的杀假丝菌素和海兔酰胺BGC。使用CORASON软件比较共线性，并使用簇中所含基因的丰度。总之，尽管这三株菌株属于同一物种，但每株都拥有独特的基因组，不同的表型（包括抗真菌和细胞外酶活性）证明了这一点。
