Biological Sciences Department, College of Sciences & Arts, King Abdulaziz University, Rabigh 21911, Saudi Arabia (Mahjoub); Department of Biomedical Science (Robbins, Greenwood), Department of Pathology and Microbiology (Galeuzzi, Graham, Jones, Buote, Conboy), Atlantic Veterinary College, University of Prince Edward Island; Canadian Wildlife Health Cooperative - Atlantic Region, Atlantic Veterinary College, University of Prince Edward Island (Galeuzzi, Graham, Jones).
Can Vet J. 2022 Jun;63(6):637-640.
To identify first-stage nematode larvae (L1) recovered from a red fox scat sample and adult female worms recovered from 2 red fox lungs at necropsy, using published molecular methods to confirm a morphological diagnosis of (French heartworm).
Red fox .
Nematode larvae recovered from a Baermann examination survey of wild canid scats ( = 101) conducted from January 2017 to August 2020, were identified by size and morphology and subjected to PCR and DNA sequencing of the small subunit (SSU) rRNA gene, the large subunit (LSU) rRNA gene, or the second internal transcribed spacer (ITS2). In addition, these techniques were applied to adult female worms recovered from the heart/lungs of 2 red foxes (obtained from PEI trappers and stored frozen at -20°C since December of 2018 and 2020).
Size and morphology of L1 recovered by Baermann examination from a wild canid scat sample (presumed to be red fox) collected near Montague, PEI and adult female worms recovered at necropsy from 2 red fox carcasses were identified as . Molecular analysis confirmed the larvae and adult worms were .
These findings indicated that has become endemic in the red fox population on PEI.
infection is potentially fatal in dogs. Veterinarians and regional diagnostic laboratories in the Maritime provinces should consider the possibility of infection in dogs with clinical signs of cardiopulmonary and/or central nervous system disease or bleeding disorders.
通过已发表的分子方法，从红狐粪便样本中回收的第一阶段线虫幼虫（L1）和剖检时从 2 只红狐肺部回收的成年雌虫，确认形态学诊断为犬恶丝虫。
红狐。
从 2017 年 1 月至 2020 年 8 月进行的野生犬科粪便贝曼氏检查调查中回收的线虫幼虫，根据大小和形态进行鉴定，并进行 PCR 和小亚基（SSU）rRNA 基因、大亚基（ LSU）rRNA 基因或第二内部转录间隔区（ITS2）的 DNA 测序。此外，这些技术还应用于从 2 只红狐（PEI 捕手获得并自 2018 年 12 月和 2020 年以来冷冻保存在-20°C）的心脏/肺部回收的成年雌虫。
从蒙塔古附近采集的疑似红狐的野生犬科粪便样本中通过贝曼氏检查回收的 L1 大小和形态以及从 2 只红狐尸体剖检时回收的成年雌虫被鉴定为犬恶丝虫。分子分析证实幼虫和成虫均为犬恶丝虫。
这些发现表明犬恶丝虫在 PEI 的红狐种群中已成为地方病。
感染在犬中是潜在致命的。海洋省份的兽医和区域诊断实验室应考虑患有心肺和/或中枢神经系统疾病或出血性疾病的犬感染的可能性。
