Department of Clinical Dentistry, Faculty of Medicine, University of Bergen, Bergen, Norway.
Oral Health Centre of Expertise in Western Norway, Vestland, Bergen, Norway.
J Contemp Dent Pract. 2021 Dec 1;22(12):1386-1392.
The aim of this study was to compare the effects of white MTA-Angelus (wMTA), Biodentine® (Biodentine) and TotalFill® BC Root Repair Material putty (TotalFill) on human dental pulp stromal cells (hDPSCs) .
hDPSCs were isolated from third molars of healthy young adults. Material elutes at different concentrations were prepared. Cells were exposed to the eluates for 1, 3, and 7 days. Cell proliferation was evaluated using 3-(4,5-dimethyl-thiazoyl)-2, 5-diphenyl-tetrazolium bromide assay. The expression of alkaline phosphatase (ALP), osteoprotegerin (OPG), osteocalcin (OC), collagen1A (Col1A), runt-related transcription factor 2 (RUNX2), vascular endothelial growth factor-A (VEGF-A), fibroblast growth factor-1 (FGF-1), interleukin 6 (IL6), tumor necrosis factor alpha (TNFα), and interleukin-1-beta (IL1β) was determined by reverse transcription-polymerase chain reaction (RT-PCR). VEGF-A protein levels and ALP activity were quantified in the culture supernatant. Data were analyzed by two-way analysis of variance (ANOVA). values <0.05 were considered statistically significant.
hDPSC proliferation was decreased in a dose-related manner for all materials on day 3. The same effect was observed with wMTA and TotalFill on day 7. RT-PCR showed that Biodentine increased the expression of the osteogenic markers ALP, OPG, and OC. TotalFill decreased the ALP expression and activity, enhanced the production of angiogenic VEGF-A, and downregulated the inflammatory IL6 on day 7.
Although the tested materials are used interchangeably in vital pulp therapy, the findings showed varied hDPSC responses. Biodentine did not affect cell proliferation and increased the expression of osteo-/odontogenic markers compared to wMTA and TotalFill, whereas TotalFill decreased cell proliferation and exhibited enhanced angiogenic and anti-inflammatory effects over time.
The clinical significance of the results needs further investigation in an attempt to provide recommendations on the selection of bioceramic pulp capping material under different scenarios of pulpal pathosis.
本研究旨在比较白色 MTA-Angelus（wMTA）、Biodentine®（Biodentine）和 TotalFill® BC 根充修复材料糊剂（TotalFill）对人牙髓基质细胞（hDPSCs）的影响。
从健康年轻成年人的第三磨牙中分离 hDPSCs。制备不同浓度的材料浸提液。细胞分别暴露于浸提液中 1、3 和 7 天。采用 3-(4,5-二甲基噻唑-2,5-二苯基四氮唑溴盐)比色法检测细胞增殖情况。采用逆转录-聚合酶链反应（RT-PCR）检测碱性磷酸酶（ALP）、护骨素（OPG）、骨钙素（OC）、胶原 1A（Col1A）、 runt 相关转录因子 2（RUNX2）、血管内皮生长因子-A（VEGF-A）、成纤维细胞生长因子-1（FGF-1）、白细胞介素 6（IL6）、肿瘤坏死因子-α（TNFα）和白细胞介素 1-β（IL1β）的表达。通过酶联免疫吸附试验（ELISA）检测培养上清液中 VEGF-A 蛋白水平和 ALP 活性。采用双因素方差分析（ANOVA）对数据进行分析。 值＜0.05 为差异有统计学意义。
所有材料在第 3 天均呈剂量依赖性抑制 hDPSC 增殖，wMTA 和 TotalFill 在第 7 天也观察到相同的效果。RT-PCR 显示，Biodentine 增加了成骨标志物 ALP、OPG 和 OC 的表达。TotalFill 在第 7 天降低了 ALP 的表达和活性，增强了血管生成 VEGF-A 的产生，下调了炎症因子 IL6。
虽然在活髓治疗中可互换使用这些测试材料，但研究结果表明 hDPSC 反应不同。与 wMTA 和 TotalFill 相比，Biodentine 不影响细胞增殖，且增加了成骨/成牙本质标志物的表达，而 TotalFill 则抑制了细胞增殖，并随着时间的推移表现出增强的血管生成和抗炎作用。
需要进一步研究这些结果的临床意义，以尝试在不同牙髓病变情况下为选择生物陶瓷盖髓材料提供建议。
