Department of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, Iran.
Department of Plant Protection, Faculty of Agriculture, University of Tabriz, Tabriz, Iran.
Syst Parasitol. 2022 Oct;99(5):545-555. doi: 10.1007/s11230-022-10044-6. Epub 2022 Jun 3.
One new and three known species of the genus Xiphinema from the rhizosphere of fruit trees and rose shrubs in East Azarbaijan province, Iran, are presented based on the morphological, morphometric and molecular characters. The new species is distinguished by its 2.0-2.1 mm long body, relatively flattened lip region with 8.7-10.0 µm width, set off from body contour by a deep constriction, odontostyle 82.5-88.0 µm long, V = 52-54, reproductive system didelphic-amphidelphic with symbiotic bacteria in the reflexed ovaries, tail conoid, dorsally convex with rounded to slightly subdigitate tip (42.0-43.5 µm long, c = 61-65, c' = 1.6-1.8), and males unknown. The new species, X. babaii sp. n., looks very close to X. californicum, and is regarded as its cryptic species, being separated from it using some morphological differences. Their separation was further corroborated using molecular data. Three known species belonging to the Xiphinema americanum group namely X. primum, X. pachtaicum and X. simile were also collected during present study, and new data were provided for them. Xiphinema simile is a new record for the Iran's nematode fauna. Molecular phylogenetic studies using partial sequences of 28S rRNA gene D2-D3 fragments were performed, and the phylogenetic relationships of the new species were discussed.
本文基于形态学、形态计量学和分子特征，介绍了来自伊朗东阿塞拜疆省果树和玫瑰丛根际的三个新种和一个已知种的属 Xiphinema。新种的特点是体长 2.0-2.1 毫米，相对扁平的唇区宽 8.7-10.0 µm，与体轮廓由深缢痕隔开，齿状突长 82.5-88.0 µm，V = 52-54，繁殖系统为双胚生殖-两性生殖，反射卵巢中带有共生细菌，尾圆锥形，背侧凸，顶端圆形至略带指状（长 42.0-43.5 µm，c = 61-65，c' = 1.6-1.8），雄虫未知。新种 X. babaii sp. n. 与 X. californicum 非常相似，被视为其隐种，通过一些形态差异与其分开。使用分子数据进一步证实了它们的分离。在本研究中还收集到了属于 Xiphinema americanum 组的三个已知种，即 X. primum、X. pachtaicum 和 X. simile，并为它们提供了新的数据。X. simile 是伊朗线虫动物区系的一个新记录。使用 28S rRNA 基因 D2-D3 片段的部分序列进行了分子系统发育研究，并讨论了新种的系统发育关系。
