Heydari F, Gharibzadeh F, Pourjam E, Pedram M
Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran.
J Helminthol. 2019 Feb 8;94:e32. doi: 10.1017/S0022149X18001232.
This contribution provides morphological and molecular data for one new and one known species of the genus Pungentus. The first species, P. azarbaijanensis n. sp., was recovered from the rhizospheric soil of grasses, collected in West Azarbaijan province, Iran, and was characterized by 2082-2365 μm long females having an angular lip region separated from the rest of the body by a constriction, 33-35 μm long odontostyle, vulva at 43.5-51.0%, 27.0-29.5 μm long rounded-conoid tail, and males unknown. It was compared morphologically with five species: P. angulosus, P. crassus, P. marietani, P. parapungens and P. pungens, which have didelphic-amphidelphic female reproductive system, body longer than 1.5 mm, and odontostyle longer than 20 μm. The second species, P. engadinensis, was recovered in three different regions of Iran (Mazandaran, Semnan and East Azarbaijan provinces). It was compared morphologically with some other populations reported all over the world. Besides morphological studies, molecular phylogenetic studies using partial sequences of 28S rDNA D2-D3 fragments were performed, and the phylogenetic relations of the two Iranian populations with other species and genera were discussed.
本论文提供了刺鼻线虫属（Pungentus）一个新物种和一个已知物种的形态学及分子数据。第一个物种，阿塞拜疆刺鼻线虫（P. azarbaijanensis）新种，从伊朗西阿塞拜疆省采集的草类根际土壤中分离得到，其特征为雌虫体长2082 - 2365μm，唇区呈角状，与虫体其余部分由缢缩分隔，口针长33 - 35μm，阴门位于虫体43.5 - 51.0%处，尾部长27.0 - 29.5μm，呈圆形 - 锥形，雄虫未知。将其与五个物种进行了形态学比较：角状刺鼻线虫（P. angulosus）、粗壮刺鼻线虫（P. crassus）、玛丽塔尼刺鼻线虫（P. marietani）、类刺鼻线虫（P. parapungens）和刺鼻线虫（P. pungens），这些物种具有双雌型 - 双阴道型雌虫生殖系统，虫体长度超过1.5mm，口针长度超过20μm。第二个物种，恩加丁刺鼻线虫（P. engadinensis），在伊朗的三个不同地区（马赞德兰省、塞姆南省和东阿塞拜疆省）被发现。将其与世界各地报道的其他一些种群进行了形态学比较。除形态学研究外，还利用28S rDNA D2 - D3片段的部分序列进行了分子系统发育研究，并讨论了这两个伊朗种群与其他物种和属之间的系统发育关系。
