一种从多头绒泡菌和裸绒泡菌这两种黏菌的原质团中分离色素的方法。

A method for separation of pigments from plasmodia of the true slime molds, Physarum polycephalum and Physarum nudum.

作者信息

Majcherczyk A, Rakoczy L, Hüttermann A

出版信息

Anal Biochem. 1987 Jan;160(1):178-83. doi: 10.1016/0003-2697(87)90628-2.

Abstract

Plasmodia of the true slime molds Physarum polycephalum and Physarum nudum were grown on agar semidefined medium (J. W. Daniel and H. Baldwin (1964) in Methods in Cell Physiology (Precsott, D. M., Ed.), Vol. 1, pp. 9-44, Academic Press, New York.) and pigments were extracted from 8-day-old plasmodia with the same solvent mixture that was used later as a mobile phase in the HPLC analysis. The separation was carried out on a strong anion exchanger column in a methanol/borate buffer solution. Under these conditions eight pigments from P. polycephalum and eight from P. nudum were revealed. This method can be used for detailed studies of plasmodial pigments in these species.

摘要

多头绒泡菌和裸绒泡菌这两种真正的黏菌的原质团在琼脂半限定培养基上生长（J.W.丹尼尔和H.鲍德温（1964年），载于《细胞生理学方法》（普雷斯科特，D.M.编），第1卷，第9 - 44页，学术出版社，纽约），并使用后来在高效液相色谱分析中用作流动相的相同溶剂混合物从8日龄的原质团中提取色素。分离在强阴离子交换柱上于甲醇/硼酸盐缓冲溶液中进行。在这些条件下，揭示了来自多头绒泡菌的8种色素和来自裸绒泡菌的8种色素。该方法可用于对这些物种中原质团色素的详细研究。