Institute of Sport Science and Innovations, Lithuanian Sports University, Kaunas, Lithuania.
Department of Health Promotion and Rehabilitation, Lithuanian Sports University, Kaunas, Lithuania.
Growth Factors. 2022 Aug;40(3-4):108-118. doi: 10.1080/08977194.2022.2083963. Epub 2022 Jun 5.
Methionine restriction (MR) reduces inflammation and increases longevity. We studied the effects of MR (0.17% kCal methionine, 10% kCal fat) and MR + high-fat diet (HFD) (0.17% methionine, 45% kCal fat) and overload-induced hypertrophy on inflammation, angiogenesis and mitochondrial activity in the hind-limb muscle in 10- and 26-month-old male C57BL/6J mice. Plasma IL-6 concentrations were higher in old compared to young mice. hypertrophy was accompanied by increased p-Akt, without a significant change in Akt and VEGF levels. In young mice on a HFD or MR + HFD diet the SDH activity was higher than in those from mice on other diets, irrespective of overload. There were no significant differences in total NAD concentration in the . MR enhanced the skeletal muscle hypertrophic response in old age that was accompanied with an increase in p-Akt without significant changes in muscle oxidative capacity, low-grade systemic inflammation, NAD, VEGF or Akt levels.
限制蛋氨酸（MR）摄入可减少炎症反应并延长寿命。我们研究了限制蛋氨酸（0.17%卡路里蛋氨酸，10%卡路里脂肪）和限制蛋氨酸+高脂肪饮食（0.17%蛋氨酸，45%卡路里脂肪）以及超负荷诱导的肌肉肥大对 10 月龄和 26 月龄雄性 C57BL/6J 小鼠后肢肌肉炎症、血管生成和线粒体活性的影响。与年轻小鼠相比，老年小鼠的血浆 IL-6 浓度更高。肥大伴随着 p-Akt 的增加，而 Akt 和 VEGF 水平没有明显变化。在高脂肪饮食或限制蛋氨酸+高脂肪饮食的年轻小鼠中，SDH 活性高于其他饮食的小鼠，而与超负荷无关。骨骼肌中总 NAD 浓度没有显著差异。MR 增强了老年时的骨骼肌肥大反应，伴随着 p-Akt 的增加，而肌肉氧化能力、低度全身炎症、NAD、VEGF 或 Akt 水平没有显著变化。
