实验感染绵羊胎盘内的弓形虫包囊

Toxoplasma gondii cysts in placentas of experimentally infected sheep.

作者信息

Dubey J P

出版信息

Am J Vet Res. 1987 Mar;48(3):352-3.

Abstract

To determine the presence of tissue cysts in ovine placentas, 6 ewes were inoculated orally with 10,000 Toxoplasma gondii oocysts at 60 days of gestation. The ewes were euthanatized and necropsied 21, 52, 56, 57, 57, and 62 days after T gondii inoculation, and placental cotyledons from each ewe were collected and homogenized. To distinguish between the presence of tachyzoites that are killed by acid pepsin solution and bradyzoites (from cysts) unaffected by this solution, a portion of each homogenate was inoculated into mice and another portion was inoculated into mice after digestion in acid pepsin solution. Toxoplasma gondii was isolated in 26 of 34 (76.4%) of mice inoculated with nondigested placentas of all 6 ewes and in 16 of 34 (47%) mice inoculated with digested placenta of 5 of 6 ewes. Seemingly, cysts do occur in placental tissue, but the digestion method was inferior, compared with the nondigestion method for recovery of T gondii from placenta.

摘要

为了确定绵羊胎盘组织囊肿的存在，在妊娠60天时，给6只母羊经口接种10,000个刚地弓形虫卵囊。在接种弓形虫后21、52、56、57、57和62天，将母羊安乐死并进行尸检，收集每只母羊的胎盘子叶并匀浆。为了区分被酸性胃蛋白酶溶液杀死的速殖子和不受该溶液影响的缓殖子（来自囊肿），将每份匀浆的一部分接种到小鼠体内，另一部分在酸性胃蛋白酶溶液中消化后接种到小鼠体内。在接种了所有6只母羊未消化胎盘的34只小鼠中，有26只（76.4%）分离出刚地弓形虫；在接种了6只母羊中5只母羊消化后胎盘的34只小鼠中，有16只（47%）分离出刚地弓形虫。看来，胎盘组织中确实存在囊肿，但与从胎盘中回收弓形虫的未消化方法相比，消化方法效果较差。