Dubey J P, Sharma S P
J Parasitol. 1980 Feb;66(1):111-4.
The distribution of Toxoplasma gondii in blood and other tissues of nine adult sheep experimentally infected with T. gondii oocysts was studied by inoculation of mice with ovine tissues. Parasitemia was detected in five sheep 6 to 11 days after inoculation (DAI) and lasting usually for 1 to 2 days. Toxoplasma gondii was isolated from numerous internal organs of five sheep killed between 7 and 28 DAI and from eight organs of a sheep killed 64 DAI. Of the three sheep killed 117, 118, and 119 DAI, T. gondii was isolated only from brain, heart, diaphragm, skeletal muscle, and intestine. Skeletal muscle was infected in all sheep. More mice became infected after inoculation with chronically infected tissues digested in 1% trypsin than undigested tissues. Results indicate that skeletal muscle and brain should be used for parasitological surveys in sheep.
通过用绵羊组织接种小鼠，研究了9只经实验感染刚地弓形虫卵囊的成年绵羊血液和其他组织中弓形虫的分布情况。接种后6至11天（接种后天数，DAI）在5只绵羊中检测到寄生虫血症，通常持续1至2天。从7至28 DAI处死的5只绵羊的许多内脏器官以及64 DAI处死的1只绵羊的8个器官中分离出弓形虫。在117、118和119 DAI处死的3只绵羊中，仅从脑、心脏、膈肌、骨骼肌和肠道中分离出弓形虫。所有绵羊的骨骼肌均被感染。与未消化的组织相比，用1%胰蛋白酶消化的慢性感染组织接种后，更多的小鼠被感染。结果表明，骨骼肌和脑应用于绵羊的寄生虫学调查。
