Ruan Yi, Xiang Ke-Fa, Zhang Hui-Min, Qin Zhen, Sun Yang, Wan Jing-Jing, Gu Wei, Liu Xia
Department of Pharmacology, School of Pharmacy, Naval Medical University (Second Military Medical University), Shanghai, 200433, China.
School of Traditional Chinese Medicine, Naval Medical University (Second Military Medical University), Shanghai, 200433, China.
BMC Sports Sci Med Rehabil. 2022 Jun 3;14(1):100. doi: 10.1186/s13102-022-00490-6.
Orosomucoid (ORM) is a positive acute phase protein verified to be upregulated in various forms of exercise-induced fatigued (EIF) rodents. However, its association with EIF among human beings remained unknown. This study aimed to explore the association between serum ORM and EIF triggered by military basic combat training (BCT).
The degree of EIF were measured by Borg's Rating of Perceived Exertion Scale (Borg-RPE-Scale®) as RPE score after BCT. Fifty-three male recruits were classified into three groups according to the RPE score: (1) group 1 (slight fatigue group): RPE score after BCT < 13; (2) group 2 (moderate fatigue group): RPE score after BCT = 13 or 14; (3) group 3 (severe fatigue group): RPE score after BCT > 14. The levels of blood ORM, lactate (LAC), cortisol and C-reactive protein (CRP) were determined before and after BCT. The diagnostic value of ORM was evaluated by receiver operating characteristic (ROC) curve analysis and logistic regression.
After BCT, the level of LAC, CRP, and cortisol increased among all groups, but the changes had no significant between-group difference (all p > 0.05). The level of ORM had a specific significant increase in group 3 (p = 0.039), and the changes of ORM (ΔORM) had significant difference among groups (p = 0.033). ROC curve analysis showed that the estimated area under ROC curve for ΔORM was 0.724 (p = 0.009) with the recommended optimal cut-off value as 0.2565 mg/mL. Logistic analysis showed that recruits with ΔORM ≥ 0.2565 mg/mL had higher odds for suffering from severe EIF, 5.625 times (95% CI 1.542-20.523, p = 0.009) as large as those with ΔORM < 0.2565 mg/mL.
ORM might be a promising biomarker of severe EIF triggered by BCT among male recruits. Its potential optimal cut-off value regarding ΔORM was recommended to be 0.2565 mg/mL.
血清类黏蛋白（ORM）是一种阳性急性期蛋白，已证实其在各种运动性疲劳（EIF）啮齿动物模型中表达上调。然而，其与人类EIF的关系尚不清楚。本研究旨在探讨血清ORM与军事基础战斗训练（BCT）引发的EIF之间的关系。
采用Borg自觉用力程度量表（Borg-RPE量表）测量BCT后的RPE评分，以此评估EIF程度。53名男性新兵根据RPE评分分为三组：（1）第1组（轻度疲劳组）：BCT后的RPE评分<13；（2）第2组（中度疲劳组）：BCT后的RPE评分为13或14；（3）第3组（重度疲劳组）：BCT后的RPE评分>14。分别测定BCT前后血液中ORM、乳酸（LAC）、皮质醇和C反应蛋白（CRP）的水平。通过受试者工作特征（ROC）曲线分析和逻辑回归评估ORM的诊断价值。
BCT后，所有组的LAC、CRP和皮质醇水平均升高，但组间变化无显著差异（均p>0.05）。第3组的ORM水平有特异性显著升高（p=0.0 .039），且ORM的变化（ΔORM）在组间有显著差异（p=0.033）。ROC曲线分析显示，ΔORM的ROC曲线下估计面积为0.724（p=0.009），推荐的最佳截断值为0.2565mg/mL。逻辑分析显示，ΔORM≥0.2565mg/mL的新兵患重度EIF的几率更高，是ΔORM<0.2565mg/mL新兵的5.625倍（95%CI 1.542-20.523，p=0.009）。
ORM可能是男性新兵中BCT引发的重度EIF的一个有前景的生物标志物。推荐其关于ΔORM的潜在最佳截断值为0.2565mg/mL。
