Eiler H, Armstrong-Backus C S, Smalling J D
Am J Vet Res. 1987 Mar;48(3):492-5.
Artificial vagina (AV) and electroejaculation (EE) are the 2 methods used to obtain semen from bulls. The purpose in the present study was to evaluate these 2 methods of collection when 2 markers, cortisol and progesterone, were injected IV. During period 1 (control measurement), semen was obtained by EE at 0, 20, 60, 120, and 180 minutes. In period 2, bulls were injected (3 days later) with a mixture of cortisol (113 mg) and progesterone (100 mg), and then samples were obtained by EE. In period 3, cortisol and progesterone were injected (3 weeks later), and samples were obtained by AV. Seminal plasma concentrations of cortisol and progesterone were maximal at 20 minutes in EE and AV collections. Seminal plasma concentrations of progesterone and cortisol were roughly 50% less in EE than in AV collection. However, the total excretion of progesterone and cortisol per collection was similar in both techniques. Excretion of cortisol was 14 to 33 times greater than that of progesterone. It was concluded that concentrations of markers in the EE were significantly less than those in AV collection. For this reason, total excretion and concentration of marker in semen should be accounted for when conducting excretion studies.
人工阴道（AV）和电射精（EE）是从公牛获取精液的两种方法。本研究的目的是评估静脉注射皮质醇和孕酮这两种标志物时的这两种采集方法。在第1阶段（对照测量），在0、20、60、120和180分钟时通过电射精获取精液。在第2阶段，公牛（3天后）注射皮质醇（113毫克）和孕酮（100毫克）的混合物，然后通过电射精获取样本。在第3阶段，注射皮质醇和孕酮（3周后），并通过人工阴道获取样本。在电射精和人工阴道采集时，精液中皮质醇和孕酮的浓度在20分钟时最高。电射精时精液中孕酮和皮质醇的浓度比人工阴道采集时低约50%。然而，两种技术每次采集时孕酮和皮质醇的总排泄量相似。皮质醇的排泄量比孕酮大14至33倍。得出的结论是，电射精时标志物的浓度明显低于人工阴道采集时。因此，在进行排泄研究时应考虑精液中标志物的总排泄量和浓度。
